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| Status |
Public on Oct 24, 2008 |
| Title |
T cell-suppression by programmed cell death 1 ligand 1(PD-L1) on IFNg-treated retinal pigment epithelial cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Adult (6- to 8-week-old) C57BL/6 purchased from CLEA Japan Inc. (Tokyo, Japan) were used as donors ocular pigment epithelium (PE). To cultivate retinal PE (RPE) eyes were cut into two parts along a circumferential line posterior to the ciliary process, creating a ciliary body-deficient posterior eyecup. The eyecup was then incubated in 0.2% trypsin (Biowhitaker) for 1 hr. These tissues were triturated to make a single cell suspension, and then re-suspended in the medium.
Keywords: Comparison
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| |
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| Overall design |
Comparison of gene expression pattern in Mouse Primary Cultured Cell Lines (RPE, RPE+IFNg).
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| Contributor(s) |
Sugita S, Futagami Y, Horie S, Aburatani H, Mochizuki M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 25, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Yuri Futagami |
| Organization name |
Tokyo Medical and Dental University
|
| Department |
Ophthalmology
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| Street address |
1-5-45 Yushima Bunkyoku
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| City |
Tokyo |
| ZIP/Postal code |
113-8519 |
| Country |
Japan |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (2) |
| GSM239614 |
Retinal pigment epithelium (RPE) |
| GSM239615 |
Retinal pigment epithelium with INFg stimulation |
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| Relations |
| BioProject |
PRJNA103173 |
