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| Status |
Public on May 23, 2017 |
| Title |
Global gene expression analysis highlights microgravity sensitive key genes in longissimus dorsi and tongue of 30 days space-flown mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Microgravity as well as chronic muscle disuse are two causes of low back pain originated at least in part from paraspinal muscle deconditioning. At present no study investigated the complexity of the molecular changes in human or mouse paraspinal muscles exposed to microgravity. The aim of this study was to evaluate longissimus dorsi and tongue (as a new potential in-flight negative control) adaptation to microgravity at global gene expression level. C57BL/N6 male mice were flown aboard the BION-M1 biosatellite for 30 days (BF) or housed in a replicate flight habitat on ground (BG). . Global gene expression analysis identified 89 transcripts differentially regulated in longissimus dorsi of BF vs. BG mice (False Discovery Rrate < 0,05 and fold change < -2 and > +2), while only a small number of genes were found differentially regulated in tongue muscle ( BF vs. BG = 27 genes).
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| Overall design |
C57BL/N6 mice were randomly divided in 3 groups: Bion Flown (BF), mice flown aboard the Bion M1 biosatellite in microgravity environment for 30 days; Bion Ground (BG), mice housed in the same habitat of flown animals but exposed to earth gravity; and Flight Control (FC), mice housed in a standard animal facility.
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| Contributor(s) |
Gambara G, Salanova M, Blottner D, Ungethüm U |
| Citation(s) |
28529490 |
| Submission date |
Feb 01, 2017 |
| Last update date |
May 04, 2018 |
| Contact name |
Dieter Blottner |
| E-mail(s) |
dieter.blottner@charite.de
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| Phone |
+49-30-450-528-347
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| Organization name |
Charité Universitaetsmedizin Berlin
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| Department |
Anatomy
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| Lab |
Neuromuscular System
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| Street address |
Phillipstrasse 12
|
| City |
Berlin |
| ZIP/Postal code |
10115 |
| Country |
Germany |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA369534 |
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