|
| Status |
Public on Feb 10, 2017 |
| Title |
Elucidation of roles of Nr4a nuclear orphan receptors and Foxp3 in thymic Treg cell development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Both Nr4a family nuclear orphan receptors and Foxp3 had been revealed to be crucial transcription factors in Treg cell development. In this study, to reveal their roles in a Treg cell developmental transcriptional programs, we compared transcriptomes among wild-type conventional CD4 T (Tconv) cells, wild-type Treg cells, Nr4a-triple-knockout (Nr4a-TKO) Treg precursor (preTreg) cells, and Foxp3-KO preTreg cells by microarray.
|
| |
|
| Overall design |
Total RNA was extracted from wild-type Tconv (CD4-single positive (CD4SP),CD25-low) thymocytes, wild-type thymic Treg (CD4SP, Foxp3+), Nr4a-TKO preTreg (CD4SP, CD25-high, GITR-high, Nr4a3-high), and Foxp3-KO preTreg (CD4SP, CD25high, Foxp3-reporter+) cells. Each cell population was analyzed with two biological replicates.
|
| |
|
| Contributor(s) |
Sekiya T, Yoshimura A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 09, 2017 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Takashi Sekiya |
| E-mail(s) |
lb-sekiya@hospk.ncgm.go.jp
|
| Organization name |
National Center for Global Health and Medicine
|
| Street address |
1-7-1 Kohnodai
|
| City |
Ichikawa |
| State/province |
Chiba |
| ZIP/Postal code |
272-0827 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA373860 |
Less...
More...