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| Status |
Public on Mar 14, 2017 |
| Title |
m6A-seq in glioblastoma stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
m6A RNA methylation plays an important role in regulating the self-renewal and tumorigenesis of glioblastoma stem cells. We performed m6A-seq analysis in glioblastoma stem cells with m6A levels modulated by knocking down METTL3 or METTL14 and identified m6A regulated genes in glioblastoma stem cells.
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| Overall design |
Glioblastoma stem cells were treated with lentivurs expressing constrol shRNA or target (METTL3 or METTL14) shRNA to knockdown the expression of METTL3 or METTL14. RNAs were extracted and m6A-seq analysis was performed using m6A specific antibody immunoprecipitation followed by RNA-seq.
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| Contributor(s) |
Cui Q, Shi H, Lu Z, He C, Shi Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
zhike lu |
| E-mail(s) |
zhikelu@gmail.com
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| Organization name |
University of Chicago
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| Department |
department of chemistry
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| Street address |
5801 South Ellis Avenue
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (8)
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| GSM2483501 |
PBT003 gliblastoma stem cells, control shRNA, Input |
| GSM2483502 |
PBT003 gliblastoma stem cells, METTL3 shRNA, Input |
| GSM2483503 |
PBT003 gliblastoma stem cells, METTL14 shRNA 1, Input |
| GSM2483504 |
PBT003 gliblastoma stem cells, METTL14 shRNA 2, Input |
| GSM2483505 |
PBT003 gliblastoma stem cells, control shRNA, m6A-IP |
| GSM2483506 |
PBT003 gliblastoma stem cells, METTL3 shRNA, m6A-IP |
| GSM2483507 |
PBT003 gliblastoma stem cells, METTL14 shRNA 1, m6A-IP |
| GSM2483508 |
PBT003 gliblastoma stem cells, METTL14 shRNA 2, m6A-IP |
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| Relations |
| BioProject |
PRJNA374490 |
| SRA |
SRP099397 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE94808_ShControl.R1.bed.gz |
129.2 Kb |
(ftp)(http) |
BED |
| GSE94808_shM14.R1.bed.gz |
171.3 Kb |
(ftp)(http) |
BED |
| GSE94808_shM14.R2.bed.gz |
158.3 Kb |
(ftp)(http) |
BED |
| GSE94808_shM3.R1.bed.gz |
127.7 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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