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| Status |
Public on Feb 17, 2017 |
| Title |
Generation of mature T cells from human hematopoietic stem/progenitor cells in artificial thymic organoids |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mechanistic studies of human T cell development require robust model systems that recapitulate the full span of thymopoiesis, from hematopoietic stem and progenitor cells (HSPC) through to mature naïve T cells. We developed a serum free, artificial thymic organoid (ATO) system that supports highly efficient and reproducible in vitro differentiation and positive selection to generate conventional human T cells from all sources of HSPCs. Deep-sequencing of the TCR variable genes was performed to compare the diversity of the TCR repertoire between ATO-derived CD8SP T cells and naive CD8SP T cells from thymus and blood.
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| Overall design |
TCR repertoire analysis of CD3+TCRab+CD8SP T cells derived from cord blood CD34+CD3- HSPCs in ATOs compared with human naïve CD8SP T cells from the thymus and blood
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| Contributor(s) |
Bethune MT, Seet CS |
| Citation(s) |
28369043 |
| Submission date |
Feb 16, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Michael T Bethune |
| Organization name |
California Institute of Technology
|
| Department |
BBE
|
| Lab |
Baltimore
|
| Street address |
1200 E California Ave, MC147-75
|
| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA374973 |
| SRA |
SRP100031 |
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