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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 10, 2017 |
| Title |
FAIRE-seq analysis of Ras transformed mouse mammary gland epithelial cells transfected with Etv4 and Etv5 siRNAs and stimulated with TGF-beta. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We performed global mapping of accessible chromatin in mouse mammary gland epithelial EpH4 cell line and its Ras-transformed derivative (EpRas) using formaldehyde-assisted isolation of regulatory element (FAIRE)-sequencing (seq) to analyze the mechanisms of transcriptional regulation during TGF-beta-induced EMT.
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| Overall design |
FAIRE-seq was perfomed using EpRas cells transfected with Etv4 and Etv5 siRNAs and stimulated with 1 ng/ml TGF-beta or left untreated for 4 days.
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| Contributor(s) |
Arase M, Isogaya K, Nakaki R, Mizutani A, Tsutsumi S, Aburatani H, Miyazono K, Koinuma D |
| Citation(s) |
28446749 |
| Submission date |
Feb 16, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Daizo Koinuma |
| E-mail(s) |
d-koinuma@umin.ac.jp
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| Organization name |
University of Tokyo
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| Department |
Pathology
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| Street address |
Hongo 7-3-1, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (4)
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| GSM2492639 |
FAIRE-seq, EpRas, siControl, untreated, 4 days |
| GSM2492640 |
FAIRE-seq, EpRas, siControl, TGF-beta treated, 4 days |
| GSM2492641 |
FAIRE-seq, EpRas, siEtv4/5 set 1, TGF-beta treated, 4 days |
| GSM2492642 |
FAIRE-seq, EpRas, siEtv4/5 set 2, TGF-beta treated, 4 days |
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| This SubSeries is part of SuperSeries: |
| GSE69387 |
Dynamics of chromatin accessibility during TGF-beta-induced EMT of Ras-transformed mammary gland epithelial cells |
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| Relations |
| BioProject |
PRJNA375002 |
| SRA |
SRP100055 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE94977_RAW.tar |
718.0 Mb |
(http)(custom) |
TAR (of BED, BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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