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Series GSE9509 Query DataSets for GSE9509
Status Public on Dec 01, 2007
Title A transcriptional repressor and co-repressor induced by the STAT3-regulated anti-inflammatory signaling pathway.
Organism Mus musculus
Experiment type Expression profiling by array
Summary IL-10 regulates anti-inflammatory signaling via the activation of STAT3, which in turn controls the induction of a gene expression program whose products execute inhibitory effects on pro-inflammatory mediator production. Here we show that IL-10 induces the expression of an ETS family transcriptional repressor, ETV3 and a helicase family co-repressor, SBNO2 (Strawberry notch homolog 2) in mouse and human macrophages. IL-10-mediated induction of ETV3 and SBNO2 expression was dependent upon both STAT3, and co-stimulus through the TLR pathway. We also observed that ETV3 expression was strongly induced by the STAT3 pathway induced by IL-10 but not STAT3 signaling activated by IL-6, which cannot activate the anti-inflammatory signaling pathway. ETV3 and SBNO2 specifically repressed NF-kB-mediated transcription and can physically interact. Collectively our data suggest that ETV3 and SBNO2 are components of the pathways that contribute to the downstream anti-inflammatory effects of IL-10.
We compared expression profiles of macrophages isolated from IL-10 -/- mice. Macrophages were treated with either LPS or LPS plus IL-10. Treatment times were 10, 20 and 30 minutes.
Keywords: time-course, treatment response, gene expression analysis
Overall design Mouse IL-10 -/- macrophages were isolated and purified and set up in culture medium containing LPS or LPS plus IL-10. A total of 18 samples were analyzed. This set contains three replicates of each treatment condition where treatment (LPS versus LPS plus IL-10) and time (10min, 20min and 30min) were varied.
Contributor(s) Murray PJ
Citation(s) 18025162
Submission date Nov 02, 2007
Last update date Aug 02, 2019
Contact name Peter J Murray
Organization name St. Jude Children's Research Hospital
Department Infectious Diseases
Street address 332 Nth Lauderdale
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (18)
GSM241128 LPS, 10 min, Rep_1
GSM241129 LPS, 10 min, Rep_2
GSM241130 LPS, 10 min, Rep_3
BioProject PRJNA103303

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Supplementary file Size Download File type/resource
GSE9509_RAW.tar 65.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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