|
| Status |
Public on Mar 28, 2008 |
| Title |
Microarray analysis of perichondral and reserve growth plate zones |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
In the growth plate, the reserve and perichondral zones have been hypothesized to have similar functions, but their exact functions are poorly understood. Our hypothesis was that significant differential gene expression exists between perichondral and reserve chondrocytes that may differentiate the respective functions of these two zones. Normal Sprague-Dawley rat growth plate chondrocytes from the perichondral zone (PC), reserve zone (RZ), proliferative zone (PZ), and hypertrophic zone (HZ) were isolated by laser microdissection and then subjected to microarray analysis. In order to most comprehensively capture the unique features of the two zones, we analyzed both the most highly expressed genes and those that were most significantly different from the proliferative zone (PZ) as a single comparator.
Keywords: cell specific expression profiles
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| |
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| Overall design |
2 microarray replicates were obtained for each zone, using pooled quantities of RNA from 3 rats each at 42 and 46 days of age.
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| |
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| Contributor(s) |
Zhang M, Pritchard MR, Middleton FA, Horton JA, Damron TA |
| Citation(s) |
18579462, 19480974, 20616531 |
| Submission date |
Nov 06, 2007 |
| Last update date |
Nov 08, 2019 |
| Contact name |
Frank A Middleton |
| E-mail(s) |
middletf@upstate.edu
|
| Phone |
315-464-7721
|
| Organization name |
SUNY Upstate Medical Univ
|
| Department |
Neuroscience and Physiology
|
| Street address |
505 Irving Avenue
|
| City |
Syracuse |
| State/province |
NY |
| ZIP/Postal code |
13210 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA103349 |
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