NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE95414 Query DataSets for GSE95414
Status Public on Oct 01, 2017
Title Expression data from human cell lines before and after treated with drugs.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Analysis of HER2-amplified NCI-N87 gastric cancer cell line exposed to 0.1 µg/mL T-DM1 and subsequently cultured in the presence of gradually increasing doses, up to a maximum of 4 µg/mL. The resultant cell lines that grew exponentially in the presence of T-DM1 were designated as drug resistant gastric cancer cell lines, and named N87-TDMR.
We used microarrays to detail the gene expression in N87-TDMR and N87-parent cells to screen for transcripts correlated with T-DM1 resistance.
 
Overall design Total RNA was purified using RNeasy Mini Kit (Qiagen), including a DNase digestion treatment. RNA concentrations were determined by NanoDrop 2000 (Thermo Scientific). RNA was labeled using the GeneChip® WT Terminal Labeling and Controls Kit combined with the Ambion® WT Expression Kit. Labeled cRNA was hybridized to GeneChip® Human Transcriptome Array 2.0 arrays (Affymetrix).
 
Contributor(s) Takegawa N, Nonagase Y, Yonesaka K, Sakai K
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Feb 27, 2017
Last update date Oct 29, 2018
Contact name Kazuko Sakai
Organization name Kindai University Faculty of Medicine
Department Dept. of Genome Biology
Street address 377-2, Osaka-sayama
City Osaka
ZIP/Postal code 589-8511
Country Japan
 
Platforms (1)
GPL17586 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]
Samples (2)
GSM2510289 Parental cell lines
GSM2510290 TDM-1 resistant cell lines
Relations
BioProject PRJNA377092

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE95414_RAW.tar 51.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap