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Series GSE9545 Query DataSets for GSE9545
Status Public on Aug 24, 2009
Title Gene Expression Profiles Associated with Ethanol-Induced Teratogenic Effects in a C57BL/6 Mouse Embryo Culture Model
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mouse model for Fetal Alcohol Syndrome. Embryos exposed to alcohol in controlled environment to assess teratogenic effects.
Fetal Alcohol Syndrome (FAS) is a leading developmental disorder. To date, a holistic view of molecular gene changes is largely unexplored. Using microarray analysis of whole embryo mouse culture with strict-control over alcohol-level, we found, directly related alcohol-metabolism, a reduction of retinol binding protein 1(Rbp1), and a, de novo expression of aldehyde dehydrogenase 1B1 (ALDH1B1). Remarkably, four key hemopoiesis genes (glycophorin A, adducin 2, beta-2 microglobulin, and ceruloplasmin) became absent, and many histone variants genes were reduced. Hypothesis-driven informatics analysis and intersection analysis of two independent experiments indicated that the altered genes are involved in cell growth, hemopoiesis, histone modification, eye and heart development, and a collective reduction in expression of growth factor genes (Igf1, Efemp1, Tieg, and Edil3) and neural specification genes (neurogenin, Sox 5, bHLHb5). Down-regulated neural specification phenotypes further supported the above findings. Further more, the gene expression profile indicated distinct subgroups which overlapped with the teratogenesis of the open- and the closed-neural tubes known in FAS. In summary, our data reveal genes alteration with causal potential for dysmorpology (e.g. retinoic acid, neuronal specification, and neurotrophic factors, and epigenetics related histone genes) and those downstream responsive genes related to alcohol metabolism, and developmental teratogenesis.
Keywords: comparison of gene expression profiles for treated vs. control
 
Overall design Comparison of whole embryo gene expression after exposure to ethanol for 46 hours. Note 2 independent experiments completed.
 
Contributor(s) Zhou FC, Zhao Q, Kuwagata M, Liu Y, Goodlett CR, McClintick JN, Liang T, Edenberg HJ, Li L
Citation(s) 18366608
Submission date Nov 07, 2007
Last update date Feb 11, 2019
Contact name Feng Zhou
Organization name Indiana University School of Medicine
Department Anatomy & Cell Biology
Street address 635 Barnhill Dr.
City Indianapolis
State/province IN
ZIP/Postal code 46202
Country USA
 
Platforms (2)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (19)
GSM241642 Embryo control EXP1 rep1
GSM241643 Embryo control EXP1 rep2
GSM241644 Embryo control EXP1 rep3
Relations
BioProject PRJNA103359

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9545_RAW.tar 115.0 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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