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| Status |
Public on Feb 22, 2018 |
| Title |
DNA methylation signature defines genes modulated by stromal cell contents of human breast tumors [MethylationEPIC] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Using genome-wide approaches we have identified groups of genes modulated by CAF-secreted factors from human breast cancer cell lines grown in different CAF-conditioned medium. The genes modulated by CAF secreted factors were characterized by a specific DNA methylation pattern: hypermethylation at transcription start site (TSS) and shore regions. Approximately 60% of them exhibited a methylation-dependent expression level and 20% of them were also dependent on the methyl-CpG-binding protein domain 2. Thus, these specific DNA methylation patterns were linked to an epigenetic control of their expression, upon CAF-secreted factors. We have therefore identified some molecular events defining the responsiveness of groups of genes to stromal cell contents in human breast tumors.
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| Overall design |
SBR3 and AU565 cell lines none treated or treated with CAF-conditionned medium or with Decitabine in duplicate
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| Contributor(s) |
Mathot P, Grandin M, Devailly G, Souaze F, Cahais V, Moran S, Campone M, Herceg Z, Esteller M, Juin P, Mehlen P, Dante R |
| Citation(s) |
29058695 |
| Submission date |
Feb 27, 2017 |
| Last update date |
Jan 20, 2019 |
| Contact name |
Robert Dante |
| E-mail(s) |
robert.dante@lyon.unicaner.fr
|
| Phone |
+33478785922
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| Organization name |
Cancer Research Center – Lyon
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| Department |
Department of Tumoral Escape
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| Street address |
28, rue Laennec
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| City |
Lyon |
| ZIP/Postal code |
69373 Lyon Cedex 08 |
| Country |
France |
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| Platforms (1) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE95462 |
DNA methylation signature defines genes modulated by stromal cell contents of human breast tumors |
|
| Relations |
| BioProject |
PRJNA377161 |
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