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Series GSE95633 Query DataSets for GSE95633
Status Public on Jul 08, 2017
Title MINC Regulates Pervasive Transcription in Yeast and Mammals
Organisms Saccharomyces cerevisiae; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Purpose: We want to know whether MINC(Mot1-Ino80C-NC2) suppress pervasive transcription at both euchromatin and heterochromatin
Using next generation sequencing we show that Mot1, Ino80 chromatin remodeling complex (Ino80C), and NC2 (MINC) colocalize on chromatin and cooperate to suppress pervasive transcription in S. cerevisiae and murine embryonic stem cells (mESCs).
Conclusion: Our ChIP-Seq and mRNA-Seq data show that MINC regulates pervasive transcription in yeast and mammals
 
Overall design Here we use ChIP-seq to identify the genome-wide location of Mot1 , Ino80 and Nc2 both in yeast and mouse. Also we use anchor away and knock out in yeast and knock down in mouse to investigate the function of MINC in regulation of pervasive transcription .
 
Contributor(s) Xue Y, Carey M, Kurdistani S
Citation(s) 28735899
Submission date Mar 02, 2017
Last update date Jul 25, 2021
Contact name Siavash K Kurdistani
E-mail(s) Skurdistani@mednet.ucla.edu
Organization name UCLA
Department Biological Chemistry
Lab Kurdistani
Street address 615 Charles E Young Dr South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (4)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL17342 Illumina HiSeq 2500 (Saccharomyces cerevisiae)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (117)
GSM2519962 WT_Rap_repeat1
GSM2519963 WT_Rap_repeat2
GSM2519964 Mot1AA_Rap_repeat1
Relations
BioProject PRJNA377731
SRA SRP101283

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE95633_Ino80KD_vs_ctr_antisense_ratio.txt.gz 5.7 Mb (ftp)(http) TXT
GSE95633_Ino80KD_vs_ctr_sense_ratio.txt.gz 10.2 Mb (ftp)(http) TXT
GSE95633_Ino80NC2KD_vs_ctr_antisense_ratio.txt.gz 6.7 Mb (ftp)(http) TXT
GSE95633_Ino80NC2KD_vs_ctr_sense_ratio.txt.gz 10.8 Mb (ftp)(http) TXT
GSE95633_Mot1Ino80KD_vs_ctr_antisense_ratio.txt.gz 6.7 Mb (ftp)(http) TXT
GSE95633_Mot1Ino80KD_vs_ctr_sense_ratio.txt.gz 10.8 Mb (ftp)(http) TXT
GSE95633_Mot1KD_vs_ctr_antisense_ratio.txt.gz 5.8 Mb (ftp)(http) TXT
GSE95633_Mot1KD_vs_ctr_sense_ratio.txt.gz 10.3 Mb (ftp)(http) TXT
GSE95633_Nc2KD_vs_ctr_antisense_ratio.txt.gz 5.7 Mb (ftp)(http) TXT
GSE95633_Nc2KD_vs_ctr_sense_ratio.txt.gz 10.3 Mb (ftp)(http) TXT
GSE95633_RAW.tar 209.1 Mb (http)(custom) TAR (of BEDGRAPH, TXT, XLSX)
GSE95633_antisense_metagene_MINCbound_3clusters.kgg.txt.gz 40.7 Kb (ftp)(http) TXT
GSE95633_mouse_MINC_overlap_at_TSS_GeneID.txt.gz 38.4 Kb (ftp)(http) TXT
GSE95633_readme_kgg_files.txt 476 b (ftp)(http) TXT
GSE95633_sense_metagene_MINCbound_2clusters.kgg.txt.gz 40.4 Kb (ftp)(http) TXT
GSE95633_yeast_MINC_overlap_at_TSS_Genename.txt.gz 6.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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