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| Status |
Public on Jun 30, 2008 |
| Title |
Identification of Phox2b-regulated genes by expression profiling of cranial motoneuron precursors on NIA 15k microarray |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Branchiomotor neurons are an important class of cranial motor neurons that innervate the branchial-arch-derived muscles of the face, jaw and neck. They arise in the ventralmost progenitor domain of the rhombencephalon characterized by expression of the homeodomain transcription factors Nkx2.2 and Phox2b. Phox2b in particular plays a key role in the specification of branchiomotor neurons. In its absence, generic neuronal differentiation is defective in the progenitor domain, and no branchiomotor neurons are produced. Conversely, ectopic expression of Phox2b in spinal regions of the neural tube promotes cell cycle exit and neuronal differentiation and at the same time induces genes and an axonal phenotype characteristic for branchiomotor neurons. How Phox2b exerts its pleiotropic functions, both as a proneural gene and a neuronal subtype determinant, has remained unknown.
To gain further insight into the genetic programme downstream of Phox2b we searched for novel Phox2b-regulated genes by cDNA microarray (here NIA 15k slides) analysis of facial branchiomotor neuron precursors from heterozygous and homozygous Phox2b mutant embryos.
Keywords: Phox2b-regulated genes identification
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| Overall design |
Four biological replicates each in dye swap
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| Contributor(s) |
Pla P, Le Crom S, Goridis C |
| Citation(s) |
18565209 |
| Submission date |
Nov 14, 2007 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Stéphane LE CROM |
| Organization name |
École normale supérieure
|
| Department |
Biology Institute - IBENS
|
| Lab |
Genomic platform
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| Street address |
46 rue d'Ulm
|
| City |
Paris |
| ZIP/Postal code |
75013 |
| Country |
France |
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| Platforms (1) |
| GPL6126 |
ENS SGDB Mus musculus NIA 15k |
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| Samples (8)
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| GSM242929 |
Phox2b LacZ+/- vs Phox2b LacZ-/- dissection 1 (NIA 15k) |
| GSM242930 |
Phox2b LacZ+/- vs Phox2b LacZ-/- dissection 1 dye swap (NIA 15k) |
| GSM242931 |
Phox2b LacZ+/- vs Phox2b LacZ-/- dissection 2 (NIA 15k) |
| GSM242932 |
Phox2b LacZ+/- vs Phox2b LacZ-/- dissection 2 dye swap (NIA 15k) |
| GSM242933 |
Phox2b LacZ+/- vs Phox2b LacZ-/- dissection 3 (NIA 15k) |
| GSM242934 |
Phox2b LacZ+/- vs Phox2b LacZ-/- dissection 3 dye swap (NIA 15k) |
| GSM242935 |
Phox2b LacZ+/- vs Phox2b LacZ-/- dissection 4 (NIA 15k) |
| GSM242936 |
Phox2b LacZ+/- vs Phox2b LacZ-/- dissection 4 dye swap (NIA 15k) |
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| This SubSeries is part of SuperSeries: |
| GSE9620 |
Identification of Phox2b-regulated genes by expression profiling of cranial motoneuron precursors |
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| Relations |
| BioProject |
PRJNA105189 |
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