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Series GSE96526 Query DataSets for GSE96526
Status Public on Mar 14, 2017
Title Expression data that were specifically regulated by lncRNA-LFAR1 in mouse primary HSCs
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Long noncoding RNAs (lncRNAs) play important roles in various biological processes; however, few have been identified that regulate hepatic stellate cells (HSCs) activation and the progression of liver fibrosis. Through a detailed analysis of the expression of lncRNAs in various tissues, we discovered the existence of a liver enriched lncRNA-LFAR1 (lncRNA-Liver Fibrosis Associated RNA1). To identify the roles of lncRNA-LFAR1 in liver fiboris, we systematically analyzed the regulation of mRNAs in primary HSCs infected with two separated lnc-LFAR1-shRNAs by RNA-seq, which revealed a panel of mRNAs that were specifically regulated by lncRNA-LFAR1 in mouse primary HSCs.
 
Overall design To identify the roles of lncRNA-LFAR1 in liver fibrosis, we systematically analyzed the regulation of mRNAs in primary HSCs infected with shRNA-control (n=2), lncRNA-shRNA1 (n=2) and lncRNA-shRNA3 (n=2) by RNA-seq.
 
Contributor(s) Hong W, Han T, Zhang K
Citation(s) 28747678
Submission date Mar 13, 2017
Last update date May 15, 2019
Contact name Kun Zhang
E-mail(s) zhangkun@tmu.edu.cn
Phone 022-83336819
Organization name Tianjin medical university
Department Department of Histology and Embryology
Street address Qixiangtai Road NO.22
City Tianjin
ZIP/Postal code 300070
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM2534811 primary HSCs infected with shRNA-control, biological rep1
GSM2534812 primary HSCs infected with shRNA-control, biological rep2
GSM2534813 primary HSCs infected with lncRNA-shRNA1, biological rep1
Relations
BioProject PRJNA378929
SRA SRP101771

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Supplementary file Size Download File type/resource
GSE96526_normalized.txt.gz 845.9 Kb (ftp)(http) TXT
GSE96526_rawcount.txt.gz 399.8 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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