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| Status |
Public on Mar 15, 2017 |
| Title |
Expression data from different B cell subsets in peripheral blood |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We found the presence of CD19hi and CD19lo B cell populations in the periphery of SLE and pemphigus patients，as well as human secondary lymphoid organs. In addition, CD19hi B cells and CD19lo B cells can be induced in vitro with the help of activated CD4+T cells.
We used microarrays to detail the global programme of gene expression of CD19hi and CD19lo B cells and identified distinct classes of up-regulated genes between them.
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| Overall design |
we established an in vitro CD4+ T and B cells co-culture system to mimic the bilateral T-B cell interaction. Purified human CD4+ T cells were stimulated in vitro with anti-CD3/CD28 Dynabeads for 24 hrs and co-cultured with autologous B cells. After 3 days, CD19hi and CD19lo B cells were purified.
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| Contributor(s) |
Liu Z, Wang Y |
| Citation(s) |
29066741 |
| Submission date |
Mar 14, 2017 |
| Last update date |
May 24, 2019 |
| Contact name |
zhicui liu |
| E-mail(s) |
lzc456123@163.com
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| Organization name |
Ruijin Hospital, Shanghai Jiao Tong University School of Medicine
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| Department |
Department of Dermatology
|
| Street address |
No. 197 Rui Jin Er Road
|
| City |
shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA379142 |
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