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Series GSE96630 Query DataSets for GSE96630
Status Public on Nov 20, 2017
Title Single-cell transcriptome analysis of EpCAM+ cells from P3.25 mouse fetal livers
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We employed marker-free single-cell RNA-Seq to characterize comprehensive transcriptional profiles of 52 EpCAM+ cells from postnatal day 3.25 mouse fetal livers. These cells show characters of cholangiocytes. Combined with our previous single cell data of E11.5~P2.5 mouse fetal livers, our data depict the dynamic trajectories with transcriptional profiles at single-cell resolution during mouse liver development, and provide insights into the fate decision and transcriptional control of self-renewal, differentiation and maturation of liver stem/progenitor cells.
 
Overall design The transcriptomes of EpCAM+ single cells from P3.25 mouse livers were analyzed in this study. ERCC Spike-ins were included in each sample as control. We analyzed 52 single cells from pooled sibling livers. All single cell samples were processed on the C1 microfluidic system.
 
Contributor(s) Su X, Shi Y, Han Z
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 15, 2017
Last update date May 15, 2019
Contact name Xianbin Su
E-mail(s) xbsu@sjtu.edu.cn
Organization name Shanghai Jiao Tong University
Department Shanghai Center for Systems Biomedicine
Street address Dongchuan Road 800
City Shanghai
ZIP/Postal code 200240
Country China
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (52)
GSM2536876 P3.25, EpCAM+ single cells, transcriptome 1
GSM2536877 P3.25, EpCAM+ single cells, transcriptome 2
GSM2536878 P3.25, EpCAM+ single cells, transcriptome 3
Relations
BioProject PRJNA379264
SRA SRP101924

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE96630_RAW.tar 24.9 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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