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Series GSE96815 Query DataSets for GSE96815
Status Public on Mar 09, 2018
Title Enhancement of Arterial Specification in Human Pluripotent Stem Cell Cultures Promotes Definitive Hematoendothelial Program with Broad Myelolymphoid Potential
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Identification of the regulators that lead to arterial specification with definitive hematopoietic potential should help to design strategies to recapitulate HSC development from human pluripotent stem cells (hPSCs). Here, using ETS1 conditional H1 hESC line, we found that ETS1 induction at the mesodermal stage of differentiation dramatically enhances the arterial specification in hPSC cultures and formation of DLL4+CXCR4+/- arterial HE with lymphoid potential and the capacity to produce red blood cells with high expression of BCL11a and b-globin. ETS1 effect was mediated through upregulation of NOTCH signaling. Together, these findings demonstrated that promotion of arterial specification in cultures could aid in generation of definitive hematopoiesis from hPSCs.
Overall design We engineered H1 human embryonic stem cells (hESC) carrying doxycycline (DOX)-inducible ETS1-P2A-EGFP transgene (iETS1-hESCs) and differentiated them to endothelial and hematopoietic cells in chemically defined conditions
Contributor(s) Slukvin I, Park MA, Swanson S, Moskvin O
Citation(s) 29791856, 29739946
Submission date Mar 20, 2017
Last update date May 21, 2019
Contact name Oleg Moskvin
Organization name University of Wisconsin-Madison
Street address 1220 Capitol Court
City Madison
State/province WI
ZIP/Postal code 53715
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (12)
GSM2544620 VE-cadherin+ endothelial cells with No Dox treatment at Day4 of Differentiation
GSM2544621 VE-cadherin+ endothelial cells with iETS1 expression (Day 2-4 of Dox) at Day4 of Differentiation
GSM2748255 DLL4- HE cells with 1.5ug/ml Dox treatment at Day5 of Differentiation
BioProject PRJNA379808
SRA SRP102181

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Supplementary file Size Download File type/resource
GSE96815_TPM_GEO.txt.gz 478.7 Kb (ftp)(http) TXT
GSE96815_counts_GEO.txt.gz 365.3 Kb (ftp)(http) TXT
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