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| Status |
Public on Aug 17, 2017 |
| Title |
Epididymal adipose tissue gene expression in wildtype mice and mice with inactivated mesoderm specific transcript |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this study is to compare transcriptome profiles of epididymal adipose tissue from wildtype mice and mice with a targeted inactivation of mesoderm specific transcript (Mest) fed a high fat diet from 8 to 16 weeks of age to gain insight for MEST function.
Gene expression profiling resulted in relatively few changes (~36 targets; FDR padj<0.1) in gene expression in epididymal WAT from wildtype mice and mice with inactivated paternal allele for Mest. A less stringent analyses of the dataset (P ≤ 0.05; with mean of ≥ 50 sequence tags for at least one group) identified 395 genes more highly expressed in epididymal adipose tissue of wildtype mice and 301 genes more highly expressed in mice with a paternal inactivation of Mest.
Gene pathway analyses of differentially expressed genes showed decreased expressed of genes associated with immune response and inflammation in high fat diet-fed mice with inactivated Mest compared with wildtype mice. Transcritome analyses supported phenotypic measurements which indicated improved glucose homeostasis in mice with inactivated Mest.
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| Overall design |
Epididymal white adipose tissue RNA was purified from wildtype mice (n=6) and mice with Mest inactivated on the paternal allele (N=5) that were fed a high fat diet (D12231; Research Diets) ad libitum from 8 to 16 weeks of age. Quality and quantity of RNAs were confirmed using an Agilent 2100 Bioanalyzer.
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| Contributor(s) |
Koza RA |
| Citation(s) |
28640866 |
| Submission date |
Mar 23, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Robert Koza |
| E-mail(s) |
rkoza@mmc.org
|
| Phone |
207-396-8313
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| Organization name |
Maine Medical Center Research Institute
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| Department |
Center for Molecular Medicine
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| Lab |
Epigenetics and Obesity Laboratory
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| Street address |
81 Research Drive
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| City |
Scarborough |
| State/province |
Maine |
| ZIP/Postal code |
04074 |
| Country |
USA |
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| Platforms (1) |
| GPL15907 |
AB 5500xl Genetic Analyzer (Mus musculus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA380237 |
| SRA |
SRP102366 |
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