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Series GSE9700 Query DataSets for GSE9700
Status Public on Nov 01, 2008
Title Thymic deletion in B10 430B
Organism Mus musculus
Experiment type Expression profiling by array
Summary Microarray analysis of thymic deletion in the B10 mouse strain
The gene expression patterns of thymocytes at the pre-selection stage and undergoing positive selection or negative selection were compared in the C57BL/10-H2k (B10k) strain. In order to sort thymocytes homogenously undergoing positive selection the 3A9 TCRHEL transgenic was used, which directs thymocyte development towards a MHC class II-restricted HEL-reactive lineage. To sort thymocytes homogenously undergoing negative selection, the 3A9 TCRHEL transgenic was crossed to the insHEL transgenic, which expresses HEL under the control of the insulin promoter in the thymic stroma, leading to efficient negative selection at the early single positive (CD4+CD8low) stage. To prepare the samples, thymic cell suspensions from female 6-8 week old B10k mice, either 3A9 TCRHEL or 3A9 TCRHEL x insHEL transgenics, were stained with CD4-FITC, CD69-PE, CD8-PerCP, and 1G12 supernatant (which recognises the 3A9 TCRHEL complex) followed by anti-IgG1-APC, and sorted. Pre-selection cells (from both transgenics) were defined as CD4+CD8+CD69-1G12low. Cells undergoing positive selection (from 3A9 TCRHEL transgenics) or negative selection (from 3A9 TCRHEL x insHEL transgenics) were defined as CD4+CD8lowCD69+1G12high.
Keywords: repeat
 
Overall design Single positive or double positive thymocytes from B10 mice were compared to those from B10 TCRHEL transgenic mice. 3 biological replicaes were used for each of the 4 different groups of thymocytes.
 
Contributor(s) Hardy K, Liston A
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Submission date Nov 27, 2007
Last update date Jan 08, 2019
Contact name Kristine Hardy
E-mail(s) kristine.hardy@anu.edu.au
Organization name University of Canberra
Lab Cytokine Gene Expression
Street address University of Canberra
City Bruce
State/province ACT
ZIP/Postal code 0200
Country Australia
 
Platforms (1)
GPL340 [MOE430B] Affymetrix Mouse Expression 430B Array
Samples (12)
GSM245239 B10 TCR single positive rep 1
GSM245240 B10 TCR single positive rep 2
GSM245241 B10 TCR single positive rep 3
Relations
BioProject PRJNA103603

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9700_RAW.tar 109.8 Mb (http)(custom) TAR (of CEL, CHP, EXP)
Processed data included within Sample table

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