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Series GSE9721 Query DataSets for GSE9721
Status Public on Dec 07, 2007
Title Expression data from flowers of two isogenic lines J10AB and J03AB at different developmental stages
Platform organism Arabidopsis thaliana
Sample organism Brassica napus
Experiment type Expression profiling by array
Summary Dominant genic male sterility (DGMS) is a valuable system for hybrid seed production and improvement of plant populations, but the molecular mechanism of DGMS is not well understood. To facilitate the analysis of mechanism underlying DGMS, we compared gene expression profiles between fertile and sterile flowers of two pair of Brassica napus isogenic lines: J10AB and J03AB which only differ in sterility-related locus and fertility restore-related locus respectively by using Arabidopsis thaliana ATH-1 microarray.
Gene expression profiling revealed that 360 genes differed in expression between the two lines and the number of differently expressed genes increased with progressing developmental stages. Cluster analysis result indicates that most important gene expression changes occur from PMC (anther less than 1mm) to uninucleate stage (anther in 3mm). There are 182 differential expressed genes detected between fertile plants and sterile plants at same stage. Some important pathways related to fertility control by KEGG analysis, such as glycolysis/gluconeogenesis pathway, flavonoid biosynthesis pathway and fatty acid metabolism pathway. Another abortion usually associated with suppression of gene expression of these pathways.
Keywords: Time course analysis
 
Overall design The Homozygous DGMS two-type line J10AB was compared with heterozygous two-type line J03AB at three different stages of development: tetrad stage, uninucleate stage and trinucleate stage (Flower buds <1, 1-3 and >3mm, respectively) which were confirmed by cytology observation. All these flower buds were collected by hand. For each stage, two biological repetitions and two experimental repetitions were used, resulting in four hybridizations per developmental stage. Gene expression was compared between lines at different stages of development, as well as between developmental stages of fertile plants and sterile plants of each line.
 
Contributor(s) Dong C, Hu S, Shi L, Zhou J, Fan Y, Liu S
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Submission date Nov 28, 2007
Last update date Aug 28, 2018
Contact name hua cai dong
E-mail(s) dongch@oilcrops.cn
Phone 86+027-86829403
Organization name Institute of Oil Crops Research of CAAS
Department Genomics and Molecular Biology
Lab Functional genomics
Street address Xudong Second Road No2
City Wuhan
State/province Hubei
ZIP/Postal code 430062
Country China
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (48)
GSM245672 small flower buds of J10A, biol_rep1
GSM245673 small flower buds of J10A, tech_rep1
GSM245674 small flower buds of J10A, biol_rep2
Relations
Affiliated with GSE69995
BioProject PRJNA103631

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9721_RAW.tar 102.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
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