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| Status |
Public on Apr 01, 2018 |
| Title |
Total RNAseq of intestinal stem cells (ISCs) and progenitor cells (EBs) with expression of Lamin Dm0 and/or UPD |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To determine the effect of Lamin Dm0 expression on the transcription profile of ISCs/EBs with and without activated Jak/Stat signalling.
Method: Lamin Dm0 and UPD were expressed in ISCs/EBs alone and in concert and the transcriptional changes compared with control flies.
Conclusions: We found that 49% of UPD induced Jak/Stat targets were normalized by Lamin Dm0 coexpression.
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| Overall design |
ISC/EB specific expression of Lamin Dm0 and/or UPD for 5 days. Isolation of ISCs/EBs by FACS and total RNA isolation.
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| Contributor(s) |
Petrovsky R, Großhans J |
| Citation(s) |
29210315 |
| Submission date |
Mar 31, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Gabriela Salinas |
| E-mail(s) |
Gabriela.Salinas-Riester@medizin.uni-goettingen.de
|
| Organization name |
Universitaetsmedizin Goettingen
|
| Department |
Department of Human Genetics
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| Lab |
NGS Integrative Genomics
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| Street address |
Justus-von-Liebig-Weg 11
|
| City |
Goettingen |
| State/province |
Lower-Saxony |
| ZIP/Postal code |
37077 |
| Country |
Germany |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA381233 |
| SRA |
SRP102852 |
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