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| Status |
Public on Sep 01, 2017 |
| Title |
Gene expression profiling of low-dose irradiated human BM-MSCs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In bone marrow (BM), there are two different types of stem/progenitor cells. With respect to hematopoiesis, hematopoietic stem/progenitor cells (HPCs) produce mature blood cells and mesenchymal stromal/stem cells (MSCs) support this. The influence of exposure to low-dose radiation on human HPCs has been investigated, and generation of both immature and mature hematopoietic cells from human HPCs is compromised. On the other hand, the influence of exposure to low-dose radiation on MSCs is not known. This gene expression profiling was created for investigation how low-dose irradiation affects BM-MSCs genomically.
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| Overall design |
BM samples from healthy adult volunteers were purchased from AllCells (Emeryville, CA). Human BM-MSCs were isolated according to our previously published methods (Yao H, Stem Cells 2014;32:2245). BM-MSCs were exposed to 0.1 Gy of γ-radiation (Cesium137) at 0.8 Gy/min by a Gammacell Irradiator (Best Theratronics, Ontario, Canada). 24 hours after irradiation, total RNA were collected from the irradiated BM-MSCs.
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| Contributor(s) |
Fujishiro A, Miura Y, Ichinohe T |
| Citation |
Title: Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cells
Authors: Aya Fujishiro, Yasuo Miura, Masaki Iwasa, Sumie Fujii, Noriko Sugino,
Akira Andoh, Hideyo Hirai, Taira Maekawa and Tatsuo Ichinohe
Journal: Inflammation and Regeneration
Issue: 37:19
DOI: 10.1186/s41232-017-0049-2
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| Submission date |
Apr 04, 2017 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Aya Fujishiro |
| E-mail(s) |
asmakino@kuhp.kyoto-u.ac.jp
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| Organization name |
Kyoto University Hospital
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| Department |
Transfusion Medicine and Cell Therapy
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| Street address |
Sakyo-ku
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| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA381533 |
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