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Series GSE97438 Query DataSets for GSE97438
Status Public on Sep 20, 2017
Title The influence of microRNAs and poly(A)-tail length on endogenous mRNA-protein complexes
Organisms Drosophila melanogaster; Homo sapiens
Experiment type Other
Summary All mRNAs are bound in vivo by proteins to form mRNA–protein complexes (mRNPs), but changes in the composition of mRNPs during post-transcriptional regulation remain largely unexplored. Here, we have analyzed, on a transcriptome-wide scale, how microRNA-mediated repression modulates the associations of core components (eIF4E, eIF4G, and PABP) and of the decay factor DDX6 in human cells. Despite the transient nature of repressed intermediates, we detected significant changes in mRNP composition, marked by dissociation of eIF4G and PABP, and by recruitment of DDX6. Furthermore, although poly(A)-tail length has been considered critical in post-transcriptional regulation, differences in steady-state tail length explained little of the variation in either PABP association or mRNP organization more generally. Instead, relative occupancy of core components correlated best with gene expression. Together, these results indicate that posttranscriptional regulatory factors influence the associations of PABP and other core factors, and do so without substantially affecting steady-state tail length.
 
Overall design Total and immunoprecipitated samples analyzed by RNA-seq and PAL-seq
 
Contributor(s) Rissland OS, Subtelny AO, Wang M, Lugowski A, Laver JD, Sidnu SS, Smibert CA, Lipshitz HD, Bartel DP
Citation(s) 29089021
Submission date Apr 05, 2017
Last update date May 15, 2019
Contact name Olivia Rissland
E-mail(s) olivia.rissland@gmail.com
Organization name The Hospital for Sick Children
Street address 686 Bay Street
City Toronto
ZIP/Postal code M5G 1X8
Country Canada
 
Platforms (3)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
Samples (34)
GSM2564602 Total RNA for PABP pUC
GSM2564603 Total RNA for PABP miR124
GSM2564604 Total RNA for PABP miR155
Relations
BioProject PRJNA381782
SRA SRP103107

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE97438_DDX6_miRNA_processed.txt.gz 447.5 Kb (ftp)(http) TXT
GSE97438_Input_for_DDX6.txt.gz 56.4 Kb (ftp)(http) TXT
GSE97438_Input_for_PABP.txt.gz 43.2 Kb (ftp)(http) TXT
GSE97438_Occupancies.txt.gz 227.8 Kb (ftp)(http) TXT
GSE97438_PABP_miRNA_processed.txt.gz 447.7 Kb (ftp)(http) TXT
GSE97438_S2_Occupancies.txt.gz 109.2 Kb (ftp)(http) TXT
GSE97438_eIF4G_miRNA_Processed.txt.gz 919.3 Kb (ftp)(http) TXT
GSE97438_miR-124_DDX6.txt.gz 61.5 Kb (ftp)(http) TXT
GSE97438_miR-124_PABP.txt.gz 40.4 Kb (ftp)(http) TXT
GSE97438_miR-155_DDX6.txt.gz 44.9 Kb (ftp)(http) TXT
GSE97438_miR-155_PABP.txt.gz 38.0 Kb (ftp)(http) TXT
GSE97438_pUC_DDX6.txt.gz 57.1 Kb (ftp)(http) TXT
GSE97438_pUC_PABP.txt.gz 44.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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