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Series GSE9758 Query DataSets for GSE9758
Status Public on Dec 04, 2007
Title Response to estradiol-ERalpha ERE Binding defective mutant
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In addition to the estrogen responsive element (ERE)-dependent gene expression, E2-ERalpha regulates transcription through functional interactions with transfactors bound to their cognate regulatory elements on DNA, hence the ERE-independent signaling pathway. However, the relative importance of the ERE-independent pathway in E2-ERalpha signaling is unclear. Our studies in infected ER-negative cell models with an ERalpha mutant (ERalpha 203/204/211E) that functions exclusively at the ERE-independent pathway demonstrated that genomic responses assessed by microarrays from the ERE-independent pathway to E2-ERalpha are not sufficient to alter cellular growth, death or motility. These findings suggest that the ERE-dependent pathway is the canonical E2-ERalpha signaling in model cell lines.
Keywords: MDA-MB-231 cells
 
Overall design Cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or cDNA for ERalpha 203/204/211E (3411E) for 48h. Infected cells were then treated with 1 nM Estradiol 17beta for 6h. All experiments were repeated six independent times conducted at at six different days
 
Contributor(s) Muyan M, Nott S, Huang Y
Citation(s) 19321454
Submission date Dec 03, 2007
Last update date Mar 25, 2019
Contact name Stephen Welle
E-mail(s) swelle@rochester.rr.com
Organization name University of Rochester
Street address 601 Elmwood Avenue
City Rochester
State/province NY
ZIP/Postal code 14642
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (12)
GSM246279 ERa3411 rep1
GSM246280 ERa3411 rep2
GSM246281 ERa3411 rep3
This SubSeries is part of SuperSeries:
GSE9761 Response to estradiol-ERalpha, estradiol-Erbeta, and ERE Binding defective mutants
Relations
BioProject PRJNA105107

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9758_RAW.tar 57.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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