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Status |
Public on May 31, 2018 |
Title |
Next Generation Sequencing for Chromatin accessbility by ATAC-seq for MDA-MB-231, MCF7 or HCT116 cell line |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
CRISPRi on PVT1 did not induce drastic changes on chromatin accessbility in MDA-MB-231 cell line. CCAT1 enhancer is accessble specifically in HCT116, not in MCF7 or MDA-MB-231.
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Overall design |
MDA-MB-231 cell line was treated non-targeting control sgRNA or PVT1 targeting sgRNA. All cell lines express dCas9-BFP-KRAB integrated via lentivirus.
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Contributor(s) |
Cho SW, Chang HY |
Citation(s) |
29731168 |
Submission date |
Apr 10, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Howard Y. Chang |
E-mail(s) |
howchang@stanford.edu
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Phone |
650-725-7022
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Organization name |
Stanford
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Lab |
Chang Lab
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Street address |
269 Campus Drive, CCSR 2130
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City |
Stanford |
State/province |
CALIFORNIA |
ZIP/Postal code |
94305 |
Country |
USA |
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Platforms (2) |
GPL15520 |
Illumina MiSeq (Homo sapiens) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (10)
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This SubSeries is part of SuperSeries: |
GSE97669 |
Promoter of lncRNA gene *PVT1* is a tumor suppressor DNA element |
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Relations |
BioProject |
PRJNA382394 |
SRA |
SRP103775 |