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| Status |
Public on Aug 28, 2017 |
| Title |
An embryonic system to assess Wnt transcriptional targets: Comparing opposing Wnt pathways and transcriptional activation and repression. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
During animal development, signals determine and organize a vast number of complex tissues using a very small number of signal transduction pathways. These developmental signaling pathways determine cell fates through a coordinated transcriptional response that remains poorly understood. The Wnt pathway is involved in a variety of these cellular functions, and its signals are transmitted in part through a β-catenin/TCF transcriptional complex. Here we report an in vivo Drosophila assay that we used to distinguish between activation, de-repression and repression of transcriptional responses, separating upstream and downstream pathway activation and canonical/non-canonical Wnt signals in embryos. We find a specific set of genes downstream of both β-catenin and TCF with an additional group of genes regulated by Wnt. The non-canonical Wnt4 regulates a separate cohort of genes. We correlate transcriptional changes with phenotypic outcomes of cell differentiation and embryo size, showing our model can be used to characterize developmental signaling compartmentalization in vivo.
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| Overall design |
2 replicates per condition
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| Contributor(s) |
Harmston N, Tolwinski NS |
| Citation(s) |
28894169 |
| Submission date |
Apr 17, 2017 |
| Last update date |
May 04, 2018 |
| Contact name |
NATHAN HARMSTON |
| E-mail(s) |
harmstonn@cardiff.ac.uk
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| Organization name |
Cardiff University
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| Street address |
Museum Avenue
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| City |
Cardiff |
| ZIP/Postal code |
CF10 3AX |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (20)
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| GSM2579478 |
tcf∆N-short embryo, biological rep2 |
| GSM2579479 |
tcf∆N-long embryo, biological rep1 |
| GSM2579480 |
tcf∆N-long embryo, biological rep2 |
| GSM2579481 |
Wg++ embryo, biological rep1 |
| GSM2579482 |
Wg++ embryo, biological rep2 |
| GSM2579483 |
arm∆N embryo, biological rep1 |
| GSM2579484 |
arm∆N embryo, biological rep2 |
| GSM2579485 |
DisArmed embryo, biological rep1 |
| GSM2579486 |
DisArmed embryo, biological rep2 |
| GSM2579487 |
Otk++ embryo, biological rep1 |
| GSM2579488 |
Otk++ embryo, biological rep2 |
| GSM2579489 |
Wnt4++ embryo, biological rep1 |
| GSM2579490 |
Wnt4++ embryo, biological rep2 |
| GSM2579491 |
arm∆N-tcf∆N-short embryo, biological rep1 |
| GSM2579492 |
arm∆N-tcf∆N-short embryo, biological rep2 |
| GSM2579493 |
Wnt4++/Otk++ embryo, biological rep1 |
| GSM2579494 |
Wnt4++/Otk++ embryo, biological rep2 |
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| Relations |
| BioProject |
PRJNA383120 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE97873_RAW.tar |
31.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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