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Series GSE9820 Query DataSets for GSE9820
Status Public on Dec 01, 2008
Title Circulating Mononuclear Cell Transcriptomes in Patients with Atherosclerotic Coronary Artery Disease
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Monocytes and T-cells play an important role in the development of atherosclerotic coronary artery disease (CAD). Differences in transcriptional activity of these cells might reflect the individual's atherosclerotic burden. Transcriptome analysis of circulating mononuclear cells from carefully matched atherosclerotic and control patients will potentially provide insights into the pathophysiology of atherosclerosis and supply biomarkers for diagnostic purposes. From patients undergoing coronary angiography because of anginal symptoms, we carefully matched 18 patients with severe triple-vessel CAD to 13 control patients without signs of CAD on angiography. All patients were on statin and aspirin treatment. RNA from circulating CD4+ T-cells, CD14+ monocytes, lipopolysaccharide-stimulated monocytes, macrophages and CD34+ progenitor cells was subjected to genome-wide expression analysis. Only CD14+ monocytes demonstrated that a small number of genes involved in activation was overexpressed in control patients, which was verified by real-time polymerase-chain reaction. In this pilot study, cautious matching of patients with severe atherosclerotic CAD with control patients without angiographic signs of coronary atherosclerosis did not reveal differences in transcriptional activity in four out of five different mononuclear cell types. In resting monocytes from patients without overt CAD some inflammatory genes were overexpressed as compared to patients with severe CAD. Large inter-individual variability prevented the use of single differentially expressed genes as biomarkers.
Keywords: disease-state analysis

 
Overall design In total 153 arrays were analyzed with 6 technical replicates (147 biological samples). CD34+ stem cells, CD4+ T-cells, resting CD14+ monocytes, stimulated monocytes and macrophages were analyzed, all from patient with severe coronary atherosclerosis or controls that had no coronary atherosclerosis as determined angiographically, and which were carefully matched for age and gender.
 
Contributor(s) Schirmer SH, Fledderus JO, Horrevoets AJ, Royen NV
Citation(s) 19059264, 20565948
Submission date Dec 07, 2007
Last update date Sep 25, 2019
Contact name Stephan Henrik Schirmer
E-mail(s) stephan.schirmer@uks.eu
Organization name Academic Medical Center
Department Cardiology
Street address Meibergdreef 9
City Amsterdam
ZIP/Postal code 1105AZ
Country Netherlands
 
Platforms (1)
GPL6255 Illumina humanRef-8 v2.0 expression beadchip
Samples (153)
GSM247703 A10_20h_P
GSM247704 A20_3h_P
GSM247705 A32_20h_C
Relations
BioProject PRJNA103779

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9820.txt.gz 36.4 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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