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Series GSE98327 Query DataSets for GSE98327
Status Public on Dec 20, 2019
Title Transcriptional changes induced by over-expression of miR-142-3p in C2C12 myoblasts.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Skeletal muscle has a central role in whole body metabolism with myofibers that represent its functional units. These are differentiated cells with different contraction power and metabolic traits. Faster contracting myofibers preferentially use glucose as substrate for energy production, while slower use lipids. Myofibers can plastically change phenotypic traits in response to pathophysiological stimuli. Since post-transcriptional mechanisms should administer the changes of these post-mitotic cells, we demonstrated that miR-142-3p influences myofiber fuel availability and mitochondrial morphology. In vitro and in vivo experiments demonstrate that miR-142-3p regulates lipids utilization. We used microarrays to characterize the global changes in gene expression in C2C12 myoblasts due to over-expression of miR-142-3p.
 
Overall design C2C12 myoblasts were transfected with miR-142-3p mimic or a scrambled sequence, as control. Transfection efficiency was estimated of about 60%. Transfection experiments were independently repeated in 6 replicates for miR-142-3p mimic and 4 replicates for scrambled sequence. Results were evaluated after 72 hours of transfection.
 
Contributor(s) Chemello F, Cagnin S, Grespi F, Martini P, Millino C, Pacchioni B, Romualdi C, Scorrano L, Lanfranchi G
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Submission date Apr 28, 2017
Last update date Jul 25, 2021
Contact name Gerolamo Lanfranchi
E-mail(s) stefano.cagnin@unipd.it
Phone +39-0498276219
Organization name University of Padova
Department CRIBI - Biotechnology Center and Biology Department
Lab Functional Genomics Lab
Street address Via U. Bassi, 58/B
City Padova
ZIP/Postal code 35131
Country Italy
 
Platforms (1)
GPL10787 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version)
Samples (10)
GSM2592314 C2C12 myoblasts transfected with miR-142-3p rep1
GSM2592315 C2C12 myoblasts transfected with miR-142-3p rep2
GSM2592316 C2C12 myoblasts transfected with miR-142-3p rep3
This SubSeries is part of SuperSeries:
GSE98328 Expression Signatures of Single Isolated Myofibers of Mouse Hindlimb and miR-27a-3p or miR-142-3p overexpression in C2C12 myoblasts
Relations
BioProject PRJNA384720

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE98327_RAW.tar 121.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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