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| Status |
Public on May 02, 2017 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes in PB- and PB-Gbx2-transfected 46C mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes in 46C mouse embryonic stem cells overexpressing PB-Gbx2 or PB empty vector at gene expression level. Results provide important information of the response of up-regulating Gbx2, such as specific mechano-responsive genes, up- or down-regulated genes that were mainly enriched in signaling pathways regulating pluripotency of stem cells
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| Overall design |
Total RNA obtained from Gbx2-overexpressed 46C ESCs compared to empty vector-transfected control 46C ESCs. Cells were cultured in serum medium supplemented with LIF.
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| Contributor(s) |
Wang M, Ye S |
| Citation(s) |
28848051 |
| Submission date |
May 01, 2017 |
| Last update date |
May 24, 2019 |
| Contact name |
Manman Wang |
| E-mail(s) |
mm-wang@ahtcm.edu.cn
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| Phone |
+8618714855575
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| Organization name |
anhui university
|
| Street address |
No 111, Jiu Long Road
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| City |
Hefei |
| ZIP/Postal code |
230601 |
| Country |
China |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA384974 |
| SRA |
SRP105772 |
