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| Status |
Public on May 11, 2017 |
| Title |
RNA-seq Analysis of Tcf1-regulated Genes in Human ESCs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose:The goal of this study is to analyze the candidate genes motified by Tcf1 to regulate the human embryonic stem cell (ESC) fate through RNA-seq approach.
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| Overall design |
HES2 human ESCs expressing PB or PB-Tcf1 were cultured in 10% KSR basal media supplemented with 10 ng/ml FGF2, 10 ng/ml Activin A and 4 µM IWR1 for two passages and then collected by using Trizol . These samples were sent to BGI (China) company to do deep sequencing by using BGISEQ-500.
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| Contributor(s) |
Ye S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 09, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
shoudong ye |
| Organization name |
Anhui University
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| Department |
Cell Biology
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| Street address |
No 111, Jiu Long Road
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| City |
Hefei |
| ZIP/Postal code |
230601 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA386022 |
| SRA |
SRP106725 |
