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| Status |
Public on May 05, 2018 |
| Title |
Overlap in Signaling through Smoothened and the Heterotrimeric G Protein G13 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA sequencing is used to compare genes regulated by a constitutively active form of Smoothened, a protein critical to Hedgehog signaling, and a constitutively active form of the alpha subunit of the heterotrimeric G proteins G13, which shares some of the primary actions of Smoothened.
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| Overall design |
Constitutively active forms of Smoothened and the alpha subunit of G13, and as a control LacZ, were introduced into C3H10T/12 cells by means of recombinant adenoviruses. RNA was isolated 48 h thereafter for sequencing.
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| Contributor(s) |
Guo X, Riobo-Del Galdo N, Kim E, Grant GR, Manning DR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 11, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
David Manning |
| E-mail(s) |
manning@mail.med.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Street address |
3620 Hamilton Walk
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA386374 |
| SRA |
SRP106916 |
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