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| Status |
Public on Feb 22, 2018 |
| Title |
Underestimation of inadvertent morpholino RNA targets: evidence from the study of Danio rerio Ser/Arg-rich splicing factors using gene editing tools |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used morpholinos and gene editing tools to inactivate the srsf5a gene. In contrast to srsf5a homozygous mutants that did not display any phenotypic traits, microinjection of sMOsrsf5a led to developmental defects. By using RNA sequencing on morphants and control embryos we were able to identify a plethora of morpholino inadvertant target.
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| Overall design |
Two biological replicates were used per conditions. Samples named CtrlMO consist in embryos injected with the control morpholino (5'-CCTCTTACCTCAGTTACAATTTATA-3', Gene Tools). Samples named sMOsrsf5a consist in embryos injected with the morpholino against srsf5a (5'-GGATTCAGTCTCACCTCTCACTGCA-3', Gene Tools).
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| |
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| Contributor(s) |
Joris M, Motte P, Muller M |
| Citation(s) |
28934490 |
| Submission date |
May 12, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Marine Joris |
| E-mail(s) |
marine.joris@doct.ulg.ac.be
|
| Organization name |
University of Liège
|
| Department |
Life Sciences
|
| Lab |
GFIMV
|
| Street address |
Chemin de la vallée 4 Quartier de la vallée 1
|
| City |
Liège |
| ZIP/Postal code |
4000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA386501 |
| SRA |
SRP107008 |
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