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| Status |
Public on May 20, 2018 |
| Title |
Sema6D reverse signaling controls lipid metabolism for macrophage polarization linking mTOR to PPARγ |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goal of this study is to compare downstream genes of Sema6D signaling in LPS plus IFNg stimulated macrophages.
Methods: Bone marrow derived macrophage mRNA profiles of 7 weeks of wild type (WT) and Sema6D-/- mice were stimulated by LPS for 4 hrs.
Results: According to this comparison, we found that 550 genes were downregulated in Sema6D-/- macrophages than WT macrophages in response to LPS.
Conclusions: Our study represents 62 genes were supressed in both M1 and M2 Sema6D-/- macrophage than WT macrophages, suggesting of Sema6D reverse sigaling genes.
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| Overall design |
Bone marrow derived macrophage mRNA profiles of 7 weeks of wild type (WT) and Sema6D-/- mice were stimulated by LPS for 4 hrs, then isolated total RNA by RNeasy kit.
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| Contributor(s) |
Kang S, Okuzaki D, Kumanogoh A |
| Citation(s) |
29777213 |
| Submission date |
May 18, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Daisuke Okuzaki |
| E-mail(s) |
dokuzaki@biken.osaka-u.ac.jp
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| Phone |
+81-6-6879-4935
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| Organization name |
Osaka univ.
|
| Department |
Immunology Frontier Research Center
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| Lab |
Human Immunology （Single Cell Genomics)
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| Street address |
Yamadaoka 3-1
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA387120 |
| SRA |
SRP107300 |
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