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| Status |
Public on Jan 25, 2018 |
| Title |
Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency [Array] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Recently, we reported that bacterial incorporation induces cellular transdifferentiation of human fibroblasts. However, the bacterium-intrinsic cellular- transdifferentiation factor remained unknown. Here, we found that cellular transdifferentiation is caused by ribosomes. Ribosomes, isolated from both prokaryotic and eukaryotic cells, induce the formation of embryoid body-like cell clusters. Numerous ribosomes are incorporated into both the cytoplasm and nucleus through trypsin-activated endocytosis, which leads to cell-cluster formation. Although ribosome-induced cell clusters (RICs) express several stemness markers and differentiate into derivatives of all three germ layers in heterogeneous cell populations, RICs fail to proliferate, alter the methylation states of pluripotent genes, or contribute to teratoma or chimera formation. However, RICs express markers of epithelial–mesenchymal transition without altering the cell cycle, despite their proliferation obstruction. These findings demonstrate that incorporation of ribosomes into host cells induces cell transdifferentiation and alters cellular plasticity.
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| Overall design |
Gene expression was measured for ribosome-induced cell clusters (RICs) and control human dermal fibroblasts (HDFs).
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| Contributor(s) |
Ito N, Katoh K, Kushige H, Saito Y, Umemoto T, Matsuzaki Y, Kiyonari H, Kobayashi D, Soga M, Yamane M, Era T, Araki N, Furuta Y, Suda T, Kida Y, Ohta K |
| Citation(s) |
29374279 |
| Submission date |
May 18, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Yutaka Saito |
| E-mail(s) |
yutaka.saito.1985@gmail.com
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| Organization name |
National Institute of Advanced Industrial Science and Technology (AIST)
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| Street address |
2-4-7 Aomi
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| City |
Koto-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
135-0064 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE99089 |
Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency |
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| Relations |
| BioProject |
PRJNA387153 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99053_RAW.tar |
18.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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