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| Status |
Public on Dec 01, 2017 |
| Title |
A Bifidobacteria-derived Tight adherence (Tad) pilus-associated protein signals colonic epithelial growth |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Development of the human gut microbiota commences at birth, with bifidobacteria being among the first colonizers of the newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization, persistence and dialogue with the host remains poorly understood. We previously identified tight adherence (Tad) pili from Bifidobacterium breve UCC2003 as an essential colonisation factor using murine models.We have identified the protein that mediates the proliferation response, and demonstrate that bifidobacteria contribute to the maturation of the naïve gut in early life through the production of specific extracellular protein structures under in vivo conditions. This bifidobacteria-derived signalling protein may represent one of the mechanisms by which members of the early colonising microbiota stimulate growth of the neonatal mucosa
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| Overall design |
Germ free Swiss Webster mice were monocolonised with Bifidobacterium with or without mutant of the bacterial gene, TadA. The treatment groups and relevant controls were; 1. Germ Free(GF) Bif UCC 2003 n=4 , 2. GF and Bif TadA (mutant) n=4, 3. GF 1 n=4, 4. GF re-conventionalised (CONV) n= 5. The colons were removed and the RNA extracted (RNAeasy plus universal kit (Qiagen), quantified and Microarrays were carried out using mouse Exon ST1.0 arrays (Affymetrix) by Almac Group, Craigavon, Northern Ireland. Analysis and pathway mapping was carried out by ALMAC and using Subio Platform software (Subio Inc) and Genesis Software.
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| Contributor(s) |
Motherway MO, MacSharry J |
| Citation missing |
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| Submission date |
May 19, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
John Andrew Mac Sharry |
| E-mail(s) |
j.macsharry@ucc.ie
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| Organization name |
UCC
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| Department |
APC, Biosciences Instiute
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| Lab |
Room 5.27/4.39
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| Street address |
College Road
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| City |
Cork |
| State/province |
Cork |
| ZIP/Postal code |
Cork1 |
| Country |
Ireland |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA387310 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99110_GeneSelectionResults_MOCM.xlsx |
4.4 Mb |
(ftp)(http) |
XLSX |
| GSE99110_RAW.tar |
349.5 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE99110_Subio_Statistics_MOCM_no_Conv_included.xlsx |
796.3 Kb |
(ftp)(http) |
XLSX |
| Processed data included within Sample table |
| Processed data are available on Series record |
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