|
| Status |
Public on May 23, 2017 |
| Title |
MicroRNA-202 maintains spermatogonial stem cells by inhibiting cell cycle regulators and RNA binding proteins |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
As miRNAs regulate gene expression either by inhibiting translation or by initiating mRNA degradation, we performed RNA sequencing to identify genes regulated by miR-202, using a Cas9/double gRNAs mediated and doxycycline induced knockout approach.
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| |
|
| Overall design |
We carried out RNA-Seq analysis of miR-202 induced knockout single clonies of spermatogonial stem cells (SSCs) with or without treatment of doxycycline. Two single clonies (iKO-SC-1 and iKO-SC-2) were included for each condition.
|
| |
|
| Contributor(s) |
Chen J, Lin X, Han C |
| Citation(s) |
27998933 |
| Submission date |
May 22, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Chunsheng Han |
| E-mail(s) |
hancs@ioz.ac.cn
|
| Phone |
86-10-64807105
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Institute of Zoology
|
| Lab |
State Key Laboratory of Reproductive Biology
|
| Street address |
1-5 Beichen West Road, Chaoyang District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (4)
|
| GSM2634655 |
iKO-SC treated with doxycycline replicate 1 |
| GSM2634656 |
iKO-SC treated without doxycycline replicate 1 |
| GSM2634657 |
iKO-SC treated with doxycycline replicate 2 |
| GSM2634658 |
iKO-SC treated without doxycycline replicate 2 |
|
| Relations |
| BioProject |
PRJNA387452 |
| SRA |
SRP107776 |
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