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| Status |
Public on Jun 13, 2008 |
| Title |
temporal profiling of retinal transcriptome regulation after IONT and IONC |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
retinal ganglion cells die after optic nerve injury, either crush or transection. The molecular causesunderlying this degeneration are largely unkwon
the purpose of this job is to find which (if any) gene regulation triggers RGC death with the final goal of design neuroprotective protocols
Keywords: time course
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| Overall design |
3 groups: naive, IONT (intraorbital nerve transection) IONC (intraorbital nerve crush). IONT and IONC lesioned animals were kept the appropriate times postlesion (12h,. 24h, 48h, 3d, 7d, and 15d). For each time point 8-12 animals were used. RNA from 4 animals was pooled and extracted to hybridaze 1 array replica. All replicas were pooled biological replicas: 5 for naive RNA (each replica 4 retinas, therefore 5 independent RNA extractions were done with a total of 20 retinas), IONT: 12h 3 replicas, 24h 2 replicas, 48h 3replicas, 3d 2 replicas, 7d 3 replicas, 15d 2 replicas. IONC: 3 replicas per time point: 12h, 24h, 48h, 3d and 7d)
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| Contributor(s) |
Agudo M, Vida-Sanz M, Sobrado P |
| Citation(s) |
18552980 |
| Submission date |
Dec 17, 2007 |
| Last update date |
Jul 31, 2017 |
| Contact name |
MARTA AGUDO |
| E-mail(s) |
martabar@um.es
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| Organization name |
MURCIA UNIVERSITY
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| Department |
OFTALMOLOGY
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| Street address |
FACULTAD MEDICINA, CAMPUS ESPINARDO
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| City |
MURCIA |
| ZIP/Postal code |
30100 |
| Country |
Spain |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (35)
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| Relations |
| BioProject |
PRJNA103915 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9918_RAW.tar |
103.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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