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Series GSE994 Query DataSets for GSE994
Status Public on Jan 27, 2004
Title Effects of cigarette smoke on the human airway epithelial cell transcriptome
Organism Homo sapiens
Experiment type Expression profiling by array
Summary A number of studies have shown that cigarette smoking produces a field defect, such that genetic mutations induced by smoking occur throughout the lung and its intra and extra-pulmonary airways. Based on this concept, we have begun this study, which has as its goal the definition of the normal airway transcriptome, an analysis of how that transcriptome is affected by cigarette smoke, and to explore the reversibility of altered gene expression when smoking has been discontinued. We have obtained brushings from intra-pulmonary airways (the right upper lobe carina) and scrapings from the buccal mucosa, from normal smoking and non-smoking volunteers (including 34 Current Smokers, 23 Never Smokers and 18 Former Smokers). RNA was isolated from these samples and gene expression profiles from intra-pulmonary airway epithelial cells were analyzed using Affymetrix U133A human gene expression arrays. All microarray data from the experiments described above have been stored, preprocessed and analyzed in a relational MySQL database that is accessible through our website at http://pulm.bumc.bu.edu/aged
Keywords = Lung
Keywords = Cancer
Keywords = Airway
Keywords = Epithelium
Keywords = Transcriptome
Keywords = Gene
Keywords = Microarray
Keywords = Bronchi
Keywords: repeat sample
 
 
Contributor(s) Spira A, Beane J, Shah V, Liu G, Schembri F, Yang X, Palma J, Brody J
Citation(s) 15210990
Submission date Jan 25, 2004
Last update date Aug 10, 2018
Contact name Avrum Spira
E-mail(s) aspira@bu.edu
Phone 617-638-4860
Fax 617-536-8093
URL http://www.pulmonomics.com
Organization name Boston University Medical Center
Department Pulmonary and Critical Care Medicine
Lab Pulmonomics Lab
Street address 715 Albany Street, R304
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (75)
GSM15684 CurrentSmoker 1
GSM15685 CurrentSmoker 2
GSM15686 CurrentSmoker 10
Relations
BioProject PRJNA88045

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Supplementary file Size Download File type/resource
GSE994_RAW.tar 238.4 Mb (http)(custom) TAR (of CEL)

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