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Status |
Public on Dec 20, 2017 |
Title |
Comprehensive Expression Analysis of microRNAs and mRNAs in Osteoarthritic Synovial Tissue from a Mouse Model of Early Post-Traumatic OA [mRNA] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Our objective was to characterize disease-specific profiles of both microRNAs (miRNAs) and mRNA expression in synovial tissue from a mouse model of early post-traumatic OA to better understand the molecular processes involved in driving OA.
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Overall design |
OA was induced in 10-12 week old male C57BL6 mice by bilateral surgical destabilization of the medial meniscus (DMM). RNA was isolated from the anterior region of the synovium of DMM and sham-operated mice at 1 and 6 weeks post-surgery. Expression profiling was performed using Agilent miRNA and mRNA microarrays and validated by qPCR. OA severity was determined histologically in the contralateral joint.
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Contributor(s) |
Kung LH, Ravi V, Rowley L, Bell KM, Little CB, Bateman JF |
Citation(s) |
29255152 |
Submission date |
Jun 06, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
John Bateman |
Organization name |
Murdoch Childrens Research Institute
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Department |
Skeletal Biology
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Lab |
John Bateman
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Street address |
Flemington Road
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City |
Melbourne |
ZIP/Postal code |
3052 |
Country |
Australia |
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Platforms (1) |
GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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Samples (16)
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This SubSeries is part of SuperSeries: |
GSE99738 |
Comprehensive Expression Analysis of microRNAs and mRNAs in Osteoarthritic Synovial Tissue from a Mouse Model of Early Post-Traumatic OA |
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Relations |
BioProject |
PRJNA389450 |