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Series GSE99800 Query DataSets for GSE99800
Status Public on Jun 07, 2020
Title Effects of Injectable DMPA and Combined Oral Contraceptive Use on HIV Susceptibility Factors in Pig-tailed Macaques
Platform organism Macaca mulatta
Sample organism Macaca nemestrina
Experiment type Expression profiling by array
Summary Progestin-based contraception may increase the risk of vaginal HIV acquisition to a level greater than the progesterone-rich luteal phase of the menstrual cycle, which has been demonstrated to have a significantly higher transmission rate compared to the follicular phase. We used pig-tailed macaque (Macaca nemestrina) model to evaluate the effects of administration of the oral the combined oral contraceptives (COCs) depot medroxyprogesterone acetate (DMPA) and levonorgestrel (LNG) on mucosal factors that influence HIV susceptibility. We compared the pH and vaginal epithelial thickness data from previous studies, and evaluated contraception-induced molecular changes in the vagina using transcriptional and cytokine profiling.
The administration of DMPA caused a pronounced thinning of the vaginal epilthelium relative to measurements takein in the follicular or luteal phase. DMPA also induced a significant increase in vaginal IL10 expression. Lastly, using RNA-Seq analyses of vaginal biopsies, we noted that both DMPA- and LNG-based contraception induced a signature of gene expression similar to that of the luteal phase, only more exacerbated, and including widespread down-regulation of HIV-restriction genes. Use of progestin-based contraception might engender a milieu that poses an increased risk of HIV transmission than that of the luteal phase via vaginal thinning, induction of immunosuppressive cytokines, and widespread suppression of HIV restriction factors.
 
Overall design 15 animals were divided into 4 sub-phases of the menstrual cycle. Vaginal pinch biopsy samples from pre-treatment (baseline) and post-HC (COC, 5 weeks after first dose; DMPA, 2 weeks post-second injection) were hybridized to Affymetrix GeneChip® Rhesus Macaque Genome Arrays.
 
Contributor(s) Vishwanathan SA, Bosinger SE, Tharp GK, Patel NB, Zhao C, Payne T, Ostergaard SD, Butler K, Mitchell J, Ellis S, Kersh EN, McNicholl JM
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Submission date Jun 08, 2017
Last update date Jul 25, 2021
Contact name Gregory K Tharp
E-mail(s) gktharp@emory.edu
Phone 404-727-7797
Organization name Yerkes National Primate Research Center
Department Developmental and Cognitive Neuroscience
Lab Genomics Core
Street address 954 Gatewood Dr
City Atlanta
State/province GA
ZIP/Postal code 30329-4208
Country USA
 
Platforms (1)
GPL3535 [Rhesus] Affymetrix Rhesus Macaque Genome Array
Samples (58)
GSM2652717 Vaginal pinch biopsy-Baseline-0.0-Ll-rep1
GSM2652718 Vaginal pinch biopsy-Baseline-0.0-Ll-rep2
GSM2652719 Vaginal pinch biopsy-Baseline-0.0-Ll-rep3
Relations
BioProject PRJNA389668

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99800_RAW.tar 277.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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