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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 01, 2017 |
| Title |
22Rv1 in vivo progression model |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Even in last stage of metastatic castration-resistant prostate cancer, androgen receptor (AR) signaling remains active.To derive high metastatic prostate cancer (PCa), we labeled AR-positive but castration-resistant 22Rv1 PCa cells with luciferase gene (22Rv1-Luc2) and these cells were orthotopically implanted in mouse prostate for spontaneous progression.
Methods: 2 × 10^5 of luciferase-expressing 22Rv1 cells (22Rv1-Luc2) cells were implanted in the anterior prostate of nude mice. After 12-14 weeks, the host mice were necropsied and the metastases from lumbar lymph nodes and primary tumors were dissected under laminar flow. Tumor tissues were minced using sterile scalpels and further digested with Collagenase D for 1 h. The lymph node metastatic cancer cells, named 22Rv1-M1, were orthotopically reimplanted in nude mice. At 12 weeks, the secondary metastases were isolated in the lumbar lymph nodes and designated as 22Rv1-M2 cells. Suspension of 1 × 10^6 22Rv1-M2 cells in DPBS was injected into nude mice through the tail vein, and mice developed metastases (22Rv1-M3) after 6 week. This procedure was repeated once to attain the 22Rv1-M4.
Results: 22Rv1-derived metastatic cell lines exhibit increased in vitro and in vivo invasion activity as the progression from 22Rv1 to M4. Transcriptomic analysis of genome-wide gene expression in the M4 tumors reveal the unique gene expression profile compared to 22Rv1 tumors.
Conclusions: Transcriptomic data provide the gene network for decoding the mechanism of PCa metastasis.
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| Overall design |
Transcriptomic profiles of the 22Rv1-Luc2 cells, 3 individual 22Rv1-derived tumor samples and 3 individual 22Rv1-M4-derived tumor samples were sequenced using RNAseq. SRA Study accession:SRP108867; BioProject accession: PRJNA389750
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| Contributor(s) |
Tsai C, Hsiao P |
| Citation(s) |
27485450 |
| BioProject |
PRJNA389750 |
| Submission date |
Jun 09, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Chin-Hsien Tsai |
| E-mail(s) |
tch@gate.sinica.edu.tw
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| Organization name |
Academia Sinica
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| Department |
ABRC
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| Street address |
Nan-Kang
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| City |
Taipei |
| State/province |
Others |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL15456 |
Illumina HiScanSQ (Homo sapiens) |
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| Samples (7)
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| Relations |
| SRA |
SRP108867 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99857_genes.fpkm_table.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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