|
| Status |
Public on Aug 05, 2013 |
| Title |
Pulmonary fibroblasts_Usual Interstitial Pneumonia_ biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Primary pulmonary fibroblasts from Usual Interstitial Pneumonia patient 2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Pulmonary fibroblasts
disease status: Usual Interstitial Pneumonia
|
| Treatment protocol |
Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
|
| Growth protocol |
Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 30007G scanner.
|
| Description |
Gene expression data from pulmonary fibroblasts in individuals with Usual Interstitial Pneumonia.
|
| Data processing |
The data were analyzed with dCHIP using the invariant set normalisation method.
|
| |
|
| Submission date |
Sep 12, 2012 |
| Last update date |
Aug 05, 2013 |
| Contact name |
Carmel Stock |
| E-mail(s) |
c.stock@imperial.ac.uk
|
| Organization name |
National Heart and Lung Institute
|
| Department |
Interstitial Lung Disease
|
| Street address |
1b Manresa Road
|
| City |
London |
| ZIP/Postal code |
SW3 6LR |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE40839 |
Expression data from fibroblasts cultured from normal and fibrotic human lung tissue |
|
