NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1003285 Query DataSets for GSM1003285
Status Public on Jan 01, 2015
Title DW292_Tg_M
Sample type RNA
 
Source name 5091212024_D
Organism Mus musculus
Characteristics strain: C57BL/6J
genotype/variation: Transgenic
gender: Male
age: embryonic day 15
tissue: frontal cortex
Treatment protocol Mouse frontal cortex was sampled from embryonic day 15 transgenic animals and littermates control using disecction kits.
Growth protocol Mice harboring a Cyfip1 spanning BAC clone (RP24-333C15) were generated in the UCLA transgenic core (http://tmc.ctrl.ucla.edu/tg-core/) by injection into C57BL/6J pronuclei. Transgenic mice were identified by PCR screening of tail derived DNA.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from embryonic day 15 frontal cortex from transgenic animals (n=3) and littermates controls (n=3) using the Qiagen RNeasy Kit. RNA quantity and quality was assessed using a Nanodrop (Nanodrop Technologies) and the Agilent Bioanalyzer (Agilent Technologies), respectively. Total RNA (200ng) was amplified, biotinylated and hybridized on Illumina Mouse ref-8 v2.0 BeadChip arrays, querying the expression of ~25,600 well-annotated Refseq transcripts, as per manufacturer's protocol.
Label Biotin
Label protocol Biotin
 
Hybridization protocol Illumina protocol
Scan protocol Illumina protocol
Data processing BeadStudio, quantile normalization
 
Submission date Sep 13, 2012
Last update date Jan 01, 2015
Contact name Giovanni Coppola
E-mail(s) gcoppola@ucla.edu
Phone 310-794-4172
Organization name UCLA
Department Psychiatry and Neurology
Lab Neurogenetics
Street address 1524 Gonda, 695 Charles Young Drive South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL6885
Series (1)
GSE40852 Overexpression of disease-associated CYFIP1 alters cellular and synaptic morphology through mTOR dysregulation of mTOR signaling

Data table header descriptions
ID_REF
VALUE Log2 transformed, quantile normalized expresssion levels

Data table
ID_REF VALUE
ILMN_1212607 6.84277192
ILMN_1212612 9.516062986
ILMN_1212619 6.658438853
ILMN_1212628 6.864957366
ILMN_1212632 7.738810512
ILMN_1212636 11.81531785
ILMN_1212637 9.315691636
ILMN_1212645 10.49382547
ILMN_1212648 9.258230386
ILMN_1212653 8.596570439
ILMN_1212672 8.819009139
ILMN_1212682 6.991991534
ILMN_1212683 6.755409895
ILMN_1212685 6.80214295
ILMN_1212692 6.787041354
ILMN_1212693 7.122278328
ILMN_1212695 6.802758558
ILMN_1212698 6.454032848
ILMN_1212702 14.36112653
ILMN_1212703 7.309934959

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap