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Sample GSM1005545 Query DataSets for GSM1005545
Status Public on Sep 18, 2012
Title ULL-D-139
Sample type RNA
 
Channel 1
Source name Stratagene Reference, ampl. totRNA
Organism Homo sapiens
Characteristics reference: Stratagene Reference, amplified total RNA
Extracted molecule total RNA
Extraction protocol not provided
Label Cy3
Label protocol not provided
 
Channel 2
Source name ULL-D-139, ampl. totRNA
Organism Homo sapiens
Characteristics cell type: primary breast carcinoma
ID: ULL-D-139
Growth protocol A Series of 212 primary breast cancer cases were studied; 80 of these tumors were analyzed using cDNA microarrays, along with one normal breast tissue sample collected from breast reduction surgery. Patient samples were sequentially collected at Ullev?l University Hospital from 1990 to 1994 (IRB approval 350, protocol 75026). The last update of patient information was done in 2006, providing an observation time of 12 to 16 years. Patients were followed until death or emigration, and only 12 patients were lost to follow-up. The average age of the 80 cases analyzed by cDNA microarrays was 65.0 years at time of primary surgery (range 28.2 to 87.7 years), similar to the average age of 64.4 years (range 28.2 to 91.5 years) for the total series. The 80 cases were selected from the total series based only on sufficient amount of fresh frozen tissue for microarray analysis. Consequently, a slightly higher fraction of patients with larger tumor size was observed in this subcohort. A summary of the clinical and histopathological data of the patients is shown in Table 1 (see Additional file 1 for more detailed information). All patients were treated according to Norwegian national guidelines at the time of diagnosis [19]. Patients receiving adjuvant systemic therapy were given nine courses of CMF (cyclophosphamide, methotrexate, 5-fluorouracil) and/or Tamoxifen for two years. Dosage of radiation given as adjuvant treatment was dependent on indication; after breast conserving therapy the mammary gland was given 50 Gy (2 Gy ? 25). The number of samples entered into the survival analyses is smaller than 212 (full dataset) and 80 (subset with gene expression data); excluding patients with missing information or distant metastases at the time of diagnosis and primary surgery, leaves us with a maximum number of 200 (full set) and 77 (subset) patients. <br> Primary breast carcinoma tissue was snap frozen and stored at -80?C. Frozen sections stained with hematoxylin/eosin were reviewed to confirm tumor content, and specimens in which at least 5% of the cells were carcinoma cells were included in this study. The majority of samples (80%) analyzed using microarrays had at least 40% tumor cell content.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from snap frozen tumor tissue using TRIzol? solution (Invitrogen?, Carlsbad, California, USA). The concentration of total RNA was determined using an HP 8453 spectrophotometer (Hewlett Packard) and the integrity of the RNA was assessed using a 2100 Bioanalyzer (Agilent, Santa Clara, California, USA).
Label Cy5
Label protocol Jeffrey Lab RNA Amplification Protocol First strand cDNA synthesis Mix the following contents in 0.2 ml PCR tube and spin briefly: N microl (3 mg) Total RNA 1 microl (0.5 mg/ml) Eberwine primer* 9-N microl RNase-free H2O * Eberwine primer = T7-oligo-dT(15) (5?-AAA CGA CGG CCA GTG AAT TGT AAT ACG ACT CAC TAT AGG CGC TTT TTT TTT TTT TTT-3?) Incubate in PCR machine at 70?C for 3 min, cool on ice for 2 min. Spin briefly. Add the following contents: 4 microl 5X first strand buffer 2 microl 0.1M DTT 1 microl RNasin 2 microl 10mM dNTP mix 2 microl Superscript?II Mix contents, spin briefly, and incubate at 42?C for 1.5 hours. Second strand cDNA synthesis Add the following contents to the first strand synthesis reaction: RNase-free H2O 106 microl 10X Advantage? PCR buffer (Clontech) 15 microl 10mM dNTP mix 3 microl RNase H (2U/microl) 1 microl Advantage? Polymerase Mix (Clontech) 3 microl Incubate in PCR machine at 37?C for 5 min, 94?C for 2 min, 65?C for 1 min, 75?C for 30 min. Stop reaction by adding 7.5 microl of 1M NaOH with 2mM EDTA and incubate in PCR machine at 65?C for 10 min. ds cDNA cleanup If necessary, transfer to a larger tube. Add 150 microl phenol:chloroform:isoamyl alcohol 25:24:1 and mix by pipetting. Transfer to phase lock gel tube and spin at ~16,000g for 5min. Transfer aqueous layer to new tube. Add 1 microl acrylamide (0.1 mg/ml), 70 microl 7.5M NH4Ac, 1 ml 100% EtOH. Spin at ~16,000g for 20 min at room temperature (not at 4?C). Wash pellet with 500 microl 75% EtOH, spin at ~16,000g for 5 min. Air dry, and suspend the pellet in 16 microl RNase-free H2O. In vitro transcription (IVT) Mix at room temperature (the spermidine in the 10X transcription buffer can coprecipitate the template DNA if the reaction is assembled on ice): 16 microl cDNA 4 microl 10X reaction buffer (room temperature) 16 microl 75mM NTP mix (on ice) 4 microl T7 enzyme mix* *Ambion T7 MEGAscript? kit Incubate at 37?C for 5 hours (we have tried incubating for 2-6 hours and achieved the highest correlation between amplified and unamplified samples at 5 hours incubation). aRNA cleanup (using Qiagen RNeasy? mini kit) Add 10 microl b-mercaptoethanol to 1ml buffer RLT before use (stable for 1 month). Transfer IVT reaction into a 1.5 ml new tube, add 60 microl RNase-free H2O, add 350 microl buffer RLT, mix by pipetting. Add 250 microl 100% EtOH, mix by pipetting. Apply sample to RNeasy? column, spin at ~12,000g for 15 sec at room temperature. Transfer column to new collection tube. Add 500 microl buffer RPE, spin at ~12,000g for 15 sec. Discard flow through, add 500 microl buffer RPE, spin at ~12,000g for 2 min. Transfer column to new collection tube, add 30 microl RNase-free H2O to the membrane, spin at ~12,000g for 1 min to elute Measure aRNA yield at A260;
 
 
Hybridization protocol 1. Prepare the reaction for each aRNA sample. aRNA Rhx Primer(8?ug/?ul) ddH2O Total Cy5 X ul(3 ug) 1 ul 16-(X+1) 16 ul Cy3 Y ul(3 ug) 1 ul 16-(Y+1) 16 ul 2. Heat to 70?C for 10 min and cool on ice. 3. Prepare reaction mixture for each Cye dye. 5X First-Strand Buffer 6 ?l 50X dNTPs 0.7 ul Cye Dyes 3 ul 0.1 M DTT 3 ul Superscript II 1.7 ul Total Volume 14.4 ul 4. Add 14.4 ul of reaction mixture to the 16 ul reaction tube, mix well, and incubate at 42?C for 1 hour. 5. Add another 1 ul of SSII to each sample and incubate at 42?C for 1 hour. 6. Add 15 ul of 0.1 N NaOH (with 2mM EDTA) and incubate at 65?C for 8 min. 7. Neutralize by adding 15 ul of 0.1 HCl. 8. Add 450 ?l of TE to a Microcon YM-30 Microcon. Add 15 ul of COT1 (1 mg/ml) DNA. 9. Add Cy5 and Cy3 labeled probe to the same Microcon. 10. Wash 1: Spin column for 7 min at 14,000 x g. Wash 2. Remove flow through and add 500 ?l TE Spin for 7 min. Wash 3. Repeat Wash 2 and concentrate probe to less than or equal to 30-35 ul. 11. Invert the Microcon into a clean 1.5 ml tube and spin for 2 min. at 14,000 RPM to recover the probe. 12. Transfer 26.1 ul of probe to new PCR tube: Sample 26.1ul Poly (A) DNA 2 ul (10 ug/ul) Yeast tRNA 1 ul (10 ug/ul) 20X SSC 5.3 ul 10% SDS 0.6 ul Total hybridization volume 35 ul 13. Denature probe by heating for 2 min at 92?C and 42?C for 20-30 min. 14. Spin briefly and place 35 ul to on array. 15. Place a coverslip (24 X 60 mm size) on to array and add 20-30 ul of 3X SSC to each corner of the hybridization chamber. 16. Hybridize at 65?C for 14-18 hours. 17. Wash slide once with 2X SSC/ 0.05% SDS, for 2 min. 18. Transfer slide to 1X SSC for 2 min. 19. Wash slide in 50?C 0.2X SSC for 2 min, twice. 20. Spin slide in 600RPM for 5min.
Scan protocol Scanner Model: GenePix 4000B
Description Simple annotation: Breast, Primary tumor
Image: http://smd.stanford.edu/MicroArray/gifs/2003-06/41879.gif
Data processing VALUE is Log (base 2) of the ratio of the median of Channel 2 (usually 635 nm) to Channel 1 (usually 532 nm)
 
Submission date Sep 18, 2012
Last update date Sep 18, 2012
Organization Stanford Microarray Database (SMD)
E-mail(s) array@genome.stanford.edu
Phone 650-498-6012
URL http://genome-www5.stanford.edu/
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL3341
Series (1)
GSE40954 TP53 mutation status and gene expression profiles are powerful prognostic markers of breast cancer

Data table header descriptions
ID_REF ID_REF
CH1I_MEAN Mean feature pixel intensity at wavelength 532 nm.; Type: integer; Scale: linear_scale
CH2I_MEAN Mean feature pixel intensity at wavelength 635 nm.; Type: integer; Scale: linear_scale
CH1B_MEDIAN The median feature background intensity at wavelength 532 nm.; Type: integer; Scale: linear_scale; Channel: Cy3 Channel; Background
CH2B_MEDIAN The median feature background intensity at wavelength 635 nm.; Type: integer; Scale: linear_scale; Channel: Cy5 channel; Background
CH1D_MEAN The mean feature pixel intensity at wavelength 532 nm with the median background subtracted.; Type: integer; Scale: linear_scale; Channel: Cy3 Channel
CH2D_MEAN .The mean feature pixel intensity at wavelength 635 nm with the median background subtracted.; Type: integer; Scale: linear_scale; Channel: Cy5 channel
CH1I_MEDIAN Median feature pixel intensity at wavelength 532 nm.; Type: integer; Scale: linear_scale
CH2I_MEDIAN Median feature pixel intensity at wavelength 635 nm.; Type: integer; Scale: linear_scale
CH1B_MEAN The mean feature background intensity at wavelength 532 nm.; Type: integer; Scale: linear_scale; Background
CH2B_MEAN The mean feature background intensity at wavelength 635 nm.; Type: integer; Scale: linear_scale; Background
CH1D_MEDIAN The median feature pixel intensity at wavelength 532 nm with the median background subtracted.; Type: integer; Scale: linear_scale
CH2D_MEDIAN The median feature pixel intensity at wavelength 635 nm with the median background subtracted.; Type: integer; Scale: linear_scale
CH1_PER_SAT The percentage of feature pixels at wavelength 532 nm that are saturated.; Type: integer; Scale: linear_scale
CH2_PER_SAT The percentage of feature pixels at wavelength 635 nm that are saturated.; Type: integer; Scale: linear_scale
CH1I_SD The standard deviation of the feature intensity at wavelength 532 nm.; Type: integer; Scale: linear_scale; Channel: Cy3 Channel
CH2I_SD The standard deviation of the feature pixel intensity at wavelength 635 nm.; Type: integer; Scale: linear_scale; Channel: Cy5 channel
CH1B_SD The standard deviation of the feature background intensity at wavelength 532 nm.; Type: float; Scale: linear_scale; Channel: Cy3 Channel; Background
CH2B_SD The standard deviation of the feature background intensity at wavelength 635 nm.; Type: integer; Scale: linear_scale; Channel: Cy5 channel; Background
PERGTBCH1I_1SD The percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength 532 nm.; Type: integer; Scale: linear_scale
PERGTBCH2I_1SD The percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength 635 nm.; Type: integer; Scale: linear_scale
PERGTBCH1I_2SD The percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength 532 nm.; Type: integer; Scale: linear_scale
PERGTBCH2I_2SD The percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength 532 nm.; Type: integer; Scale: linear_scale
SUM_MEAN The sum of the arithmetic mean intensities for each wavelength, with the median background subtracted.; Type: integer; Scale: linear_scale
SUM_MEDIAN The sum of the median intensities for each wavelength, with the median background subtracted.; Type: integer; Scale: linear_scale
RAT1_MEAN Ratio of the arithmetic mean intensities of each spot for each wavelength, with the median background subtracted. Channel 1/Channel 2 ratio, (CH1I_MEAN - CH1B_MEDIAN)/(CH2I_MEAN - CH2B_MEDIAN) or Green/Red ratio.; Type: float; Scale: linear_scale
RAT2_MEAN The ratio of the arithmetic mean intensities of each feature for each wavelength, with the median background subtracted.; Type: float; Scale: linear_scale
RAT2_MEDIAN The ratio of the median intensities of each feature for each wavelength, with the median background subtracted.; Type: float; Scale: linear_scale
PIX_RAT2_MEAN The geometric mean of the pixel-by-pixel ratios of pixel intensities, with the median background subtracted.; Type: float; Scale: linear_scale
PIX_RAT2_MEDIAN The median of pixel-by-pixel ratios of pixel intensities, with the median background subtracted.; Type: float; Scale: linear_scale
RAT2_SD The geometric standard deviation of the pixel intensity ratios.; Type: float; Scale: linear_scale
TOT_SPIX The total number of feature pixels.; Type: integer; Scale: linear_scale
TOT_BPIX The total number of background pixels.; Type: integer; Scale: linear_scale
REGR The regression ratio of every pixel in a 2-feature-diameter circle around the center of the feature.; Type: float; Scale: linear_scale
CORR The correlation between channel1 (Cy3) & Channel 2 (Cy5) pixels within the spot, and is a useful quality control parameter. Generally, high values imply better fit & good spot quality.; Type: float; Scale: linear_scale
DIAMETER The diameter in um of the feature-indicator.; Type: integer; Scale: linear_scale
X_COORD X-coordinate of the center of the spot-indicator associated with the spot, where (0,0) is the top left of the image.; Type: integer; Scale: linear_scale
Y_COORD Y-coordinate of the center of the spot-indicator associated with the spot, where (0,0) is the top left of the image.; Type: integer; Scale: linear_scale
TOP Box top: int(((centerX - radius) - Xoffset) / pixelSize).; Type: integer; Scale: linear_scale
BOT Box bottom: int(((centerX + radius) - Xoffset) / pixelSize).; Type: integer; Scale: linear_scale
LEFT Box left: int(((centerY - radius) - yoffset) / pixelSize).; Type: integer; Scale: linear_scale
RIGHT Box right: int(((centerY + radius) - yoffset) / pixelSize); Type: integer; Scale: linear_scale
FLAG The type of flag associated with a feature: -100 = user-flagged null spot; -50 = software-flagged null spot; 0 = spot valid.; Type: integer; Scale: linear_scale
CH2IN_MEAN Normalized value of mean Channel 2 (usually 635 nm) intensity (CH2I_MEAN/Normalization factor).; Type: integer; Scale: linear_scale; Channel: Cy5 channel
CH2BN_MEDIAN Normalized value of median Channel 2 (usually 635 nm) background (CH2B_MEDIAN/Normalization factor).; Type: integer; Scale: linear_scale; Channel: Cy5 channel; Background
CH2DN_MEAN Normalized value of mean Channel 2 (usually 635 nm) intensity with normalized background subtracted (CH2IN_MEAN - CH2BN_MEDIAN).; Type: integer; Scale: linear_scale; Channel: Cy5 channel
RAT2N_MEAN Type: float; Scale: linear_scale
CH2IN_MEDIAN Normalized value of median Channel 2 (usually 635 nm) intensity (CH2I_MEDIAN/Normalization factor).; Type: integer; Scale: linear_scale
CH2DN_MEDIAN Normalized value of median Channel 2 (usually 635 nm) intensity with normalized background subtracted (CH2IN_MEDIAN - CH2BN_MEDIAN).; Type: integer; Scale: linear_scale
RAT1N_MEAN Ratio of the means of Channel 1 (usually 532 nm) intensity to normalized Channel 2 (usually 635 nm) intensity with median background subtracted (CH1D_MEAN/CH2DN_MEAN). Channel 1/Channel 2 ratio normalized or Green/Red ratio normalized.; Type: float; Scale: linear_scale
RAT2N_MEDIAN Channel 2/Channel 1 ratio normalized, RAT2_MEDIAN/Normalization factor or Red/Green median ratio normalized.; Type: float; Scale: linear_scale
LOG_RAT2N_MEAN Log (base 2) of the ratio of the mean of Channel 2 (usually 635 nm) to Channel 1 (usually 532 nm) [log (base 2) (RAT2N_MEAN)].; Type: float; Scale: log_base_2
VALUE Log (base 2) of the ratio of the median of Channel 2 (usually 635 nm) to Channel 1 (usually 532 nm) [log (base 2) (RAT2N_MEDIAN)].; Type: float; Scale: log_base_2

Data table
ID_REF CH1I_MEAN CH2I_MEAN CH1B_MEDIAN CH2B_MEDIAN CH1D_MEAN CH2D_MEAN CH1I_MEDIAN CH2I_MEDIAN CH1B_MEAN CH2B_MEAN CH1D_MEDIAN CH2D_MEDIAN CH1_PER_SAT CH2_PER_SAT CH1I_SD CH2I_SD CH1B_SD CH2B_SD PERGTBCH1I_1SD PERGTBCH2I_1SD PERGTBCH1I_2SD PERGTBCH2I_2SD SUM_MEAN SUM_MEDIAN RAT1_MEAN RAT2_MEAN RAT2_MEDIAN PIX_RAT2_MEAN PIX_RAT2_MEDIAN RAT2_SD TOT_SPIX TOT_BPIX REGR CORR DIAMETER X_COORD Y_COORD TOP BOT LEFT RIGHT FLAG CH2IN_MEAN CH2BN_MEDIAN CH2DN_MEAN RAT2N_MEAN CH2IN_MEDIAN CH2DN_MEDIAN RAT1N_MEAN RAT2N_MEDIAN LOG_RAT2N_MEAN VALUE
1 695 700 197 158 498 542 702 713 204 163 505 555 0 0 226 232 80 60 96 96 96 96 1040 1060 .919 1.088 1.099 1.081 1.093 1.28 32 171 1.041 .93 60 1950 8280 81 87 61 67 0 748 169 579 1.163 762 593 .86 1.174 .218 .232
2 6541 1156 190 145 6351 1011 6843 1182 195 147 6653 1037 0 0 3838 626 48 43 100 96 100 96 7362 7690 6.282 .159 .156 .165 .159 1.221 52 276 .159 .99 80 2100 8280 80 88 75 83 0 1235 155 1080 .17 1263 1108 5.879 .167 -2.556 -2.586
3 2078 1185 189 137 1889 1048 1177 890 189 139 988 753 0 0 2396 860 35 39 100 100 100 100 2937 1741 1.802 .555 .762 .674 .702 1.393 12 104 .401 .91 50 2240 8270 80 85 90 95 0 1266 146 1120 .593 951 805 1.687 .814 -.754 -.296
4 909 610 183 146 726 464 899 605 184 145 716 459 0 0 395 220 37 40 100 100 100 100 1190 1175 1.565 .639 .641 .683 .656 1.407 12 104 .583 .93 50 2380 8270 80 85 104 109 0 652 156 496 .683 646 490 1.464 .685 -.55 -.546
5 5672 3458 186 144 5486 3314 6239 3664 262 174 6053 3520 0 0 2691 1489 227 151 98 100 98 98 8800 9573 1.655 .604 .582 .644 .605 1.367 52 314 .592 .99 90 2530 8280 79 88 117 126 0 3695 154 3541 .645 3915 3761 1.549 .621 -.632 -.687
6 652 462 177 134 475 328 645 455 180 135 468 321 0 0 227 181 32 34 100 96 93 93 803 789 1.448 .691 .686 .674 .726 1.518 32 170 .691 .93 60 2670 8280 81 87 133 139 0 494 143 350 .738 486 343 1.355 .733 -.439 -.448
7 2992 1860 179 133 2813 1727 2949 1743 181 134 2770 1610 0 0 1698 1007 32 33 100 100 100 100 4540 4380 1.629 .614 .581 .632 .609 1.25 52 300 .601 .98 90 2820 8280 79 88 146 155 0 1987 142 1845 .656 1862 1720 1.524 .621 -.608 -.687
8 783 1153 173 124 610 1029 604 835 173 128 431 711 0 0 479 844 25 32 96 100 94 98 1639 1142 .593 1.687 1.65 1.749 1.675 1.525 52 265 1.745 .97 80 2970 8280 80 88 162 170 0 1232 132 1099 1.802 892 760 .555 1.763 .85 .818
9 931 630 172 126 759 504 914 627 173 129 742 501 0 0 267 178 27 33 100 100 100 100 1263 1243 1.506 .664 .675 .668 .643 1.357 52 292 .651 .95 90 3120 8280 79 88 176 185 0 673 135 539 .709 670 535 1.409 .721 -.495 -.471
10 897 667 173 134 724 533 878 635 176 133 705 501 0 0 321 252 28 34 100 100 100 96 1257 1206 1.358 .736 .711 .725 .743 1.263 32 170 .749 .96 60 3260 8280 81 87 192 198 0 713 143 569 .787 678 535 1.271 .759 -.346 -.397
11 3837 5900 169 120 3668 5780 3701 5725 168 124 3532 5605 0 0 1280 1891 26 31 100 100 100 100 9448 9137 .635 1.576 1.587 1.593 1.582 1.119 52 280 1.567 .99 80 3410 8290 81 89 206 214 0 6304 128 6176 1.684 6117 5989 .594 1.696 .752 .762
12 3402 3714 176 131 3226 3583 1219 1280 212 139 1043 1149 0 0 4268 4477 164 51 75 90 58 80 6809 2192 .9 1.111 1.102 1.149 1.143 1.731 80 276 1.069 .99 100 3570 8280 79 89 221 231 0 3968 140 3828 1.187 1368 1228 .843 1.177 .247 .235
13 2819 1611 188 144 2631 1467 1971 1320 221 149 1783 1176 0 0 2209 1201 157 48 98 100 92 97 4098 2959 1.793 .558 .66 .561 .56 1.303 80 292 .546 .99 100 3690 8290 80 90 233 243 0 1721 154 1567 .596 1410 1257 1.679 .705 -.747 -.505
14 1016 1095 180 136 836 959 945 966 184 138 765 830 0 0 357 431 30 34 100 100 100 100 1795 1595 .872 1.147 1.085 1.165 1.14 1.342 52 289 1.183 .97 90 3850 8280 79 88 249 258 0 1170 145 1025 1.226 1032 887 .816 1.159 .294 .213
15 1275 1586 171 131 1104 1455 1372 1545 168 133 1201 1414 0 0 370 464 48 41 100 100 98 100 2559 2615 .759 1.318 1.177 1.36 1.315 1.291 52 266 1.303 .98 80 3990 8280 80 88 264 272 0 1695 140 1555 1.408 1651 1511 .71 1.258 .494 .331
16 11316 5148 176 131 11140 5017 11338 4930 169 130 11162 4799 0 0 4201 2049 46 40 100 100 100 100 16157 15961 2.22 .45 .43 .452 .448 1.15 52 269 .454 .99 80 4140 8290 81 89 279 287 0 5500 140 5360 .481 5268 5128 2.078 .459 -1.055 -1.122
17 3046 5546 176 131 2870 5415 3078 5377 176 133 2902 5246 0 0 1502 2812 27 36 100 100 100 100 8285 8148 .53 1.887 1.808 1.936 1.896 1.172 52 235 1.884 .99 80 4290 8290 81 89 294 302 0 5926 140 5786 2.016 5745 5605 .496 1.931 1.011 .95
18 194 168 168 128 26 40 188 144 169 129 20 16 0 0 52 83 27 29 42 40 17 25 66 36 .65 1.538 .8 .901 1 3.065 52 223 1.701 .6 80 4420 8280 80 88 307 315 -50 180 137 43 1.644 154 17 .608 .855 .717 -.226
19 4196 6235 167 128 4029 6107 4619 6574 170 133 4452 6446 0 0 2374 3452 34 40 100 100 98 100 10136 10898 .66 1.516 1.448 1.613 1.545 1.291 80 315 1.501 .99 100 4570 8290 80 90 321 331 0 6662 137 6525 1.62 7024 6887 .617 1.547 .696 .629
20 4442 4662 170 123 4272 4539 4318 4359 171 127 4148 4236 0 0 1534 1581 35 40 98 100 98 100 8811 8384 .941 1.063 1.021 1.109 1.035 1.343 52 284 1.054 .99 90 4720 8290 80 89 336 345 0 4981 131 4850 1.135 4657 4526 .881 1.091 .183 .126

Total number of rows: 43104

Table truncated, full table size 9620 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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