|
| Status |
Public on Sep 20, 2012 |
| Title |
Vehicle Input |
| Sample type |
SRA |
| |
|
| Source name |
Input RNA
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Hypothalamus
strain: C57Bl/6J
treatment: IP injection of PBS (325 uL) 2h before sacrifice
ps6 antibody: none
|
| Treatment protocol |
Mice were killed by cervical dislocation at approximately CT6 and hypothalami rapidly dissected.
|
| Growth protocol |
Wild-type C57Bl/6J mice were maintained on a 12 hour light-dark cycle and fed ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Immunoprecipitated and total RNA was purified using the RNAeasy Micro kit.
Clontech SMARTER + Illumina TruSeq RNA-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
SCS-RTA: On-board image analysis
CASAVA v1.8 : Bcl to fastq converstion
Tophat v1.3.1 (Bowtie 0.12.7): Junction mapping/aligning
Cufflinks/Cuffdiff v1.3.0: Transcript assembly/quantitation
Genome build: mm9
|
| |
|
| Submission date |
Sep 18, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Zachary Knight |
| Organization name |
Rockefeller University
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE40969 |
Molecular profiling of activated neurons by phosphorylated ribosome capture [RNA-Seq] |
| GSE40995 |
Molecular profiling of activated neurons by phosphorylated ribosome capture |
|
| Relations |
| SRA |
SRX188582 |
| BioSample |
SAMN01179331 |
