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Sample GSM1006146 Query DataSets for GSM1006146
Status Public on Sep 20, 2012
Title Vehicle IP
Sample type SRA
 
Source name pS6 244 immunoprecipitate RNA
Organism Mus musculus
Characteristics tissue: Hypothalamus
strain: C57Bl/6J
treatment: IP injection of PBS (325 uL) 2h before sacrifice
ps6 antibody: Cell Signaling #2215 lot 10 + 750 nM 3P phosphopeptide
Treatment protocol Mice were killed by cervical dislocation at approximately CT6 and hypothalami rapidly dissected.
Growth protocol Wild-type C57Bl/6J mice were maintained on a 12 hour light-dark cycle and fed ad libitum.
Extracted molecule total RNA
Extraction protocol Immunoprecipitated and total RNA was purified using the RNAeasy Micro kit.
Clontech SMARTER + Illumina TruSeq RNA-seq
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing SCS-RTA: On-board image analysis
CASAVA v1.8 : Bcl to fastq converstion
Tophat v1.3.1 (Bowtie 0.12.7): Junction mapping/aligning
Cufflinks/Cuffdiff v1.3.0: Transcript assembly/quantitation
Genome build: mm9
 
Submission date Sep 18, 2012
Last update date May 15, 2019
Contact name Zachary Knight
Organization name Rockefeller University
Street address 1230 York Ave
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL13112
Series (2)
GSE40969 Molecular profiling of activated neurons by phosphorylated ribosome capture [RNA-Seq]
GSE40995 Molecular profiling of activated neurons by phosphorylated ribosome capture
Relations
SRA SRX188583
BioSample SAMN01179332

Supplementary file Size Download File type/resource
GSM1006146_749.combined.accepted_hits.bam.wig.gz 16.5 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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