NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1006147 Query DataSets for GSM1006147
Status Public on Sep 20, 2012
Title Salt Input
Sample type SRA
 
Source name Input RNA
Organism Mus musculus
Characteristics tissue: Hypothalamus
strain: C57Bl/6J
treatment: IP injection of 2M NaCl (325 uL) 2h before sacrifice
ps6 antibody: none
Treatment protocol Mice were killed by cervical dislocation at approximately CT6 and hypothalami rapidly dissected.
Growth protocol Wild-type C57Bl/6J mice were maintained on a 12 hour light-dark cycle and fed ad libitum.
Extracted molecule total RNA
Extraction protocol Immunoprecipitated and total RNA was purified using the RNAeasy Micro kit.
Clontech SMARTER + Illumina TruSeq RNA-seq
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing SCS-RTA: On-board image analysis
CASAVA v1.8 : Bcl to fastq converstion
Tophat v1.3.1 (Bowtie 0.12.7): Junction mapping/aligning
Cufflinks/Cuffdiff v1.3.0: Transcript assembly/quantitation
Genome build: mm9
 
Submission date Sep 18, 2012
Last update date May 15, 2019
Contact name Zachary Knight
Organization name Rockefeller University
Street address 1230 York Ave
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL13112
Series (2)
GSE40969 Molecular profiling of activated neurons by phosphorylated ribosome capture [RNA-Seq]
GSE40995 Molecular profiling of activated neurons by phosphorylated ribosome capture
Relations
SRA SRX188584
BioSample SAMN01179333

Supplementary file Size Download File type/resource
GSM1006147_774.combined.accepted_hits.bam.wig.gz 15.1 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap