|
| Status |
Public on Nov 08, 2013 |
| Title |
Dietary Restriction 4 |
| Sample type |
RNA |
| |
|
| Source name |
Dietary Restriction 4
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
strain: C57BL6/J
age: 8 months
tissue: Liver
Sex: Male
protocol: 40% Dietary Restriction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 25 mg of liver in each sample using Qiagen Rneasy kit following manufacturer’s protocol. RNA quantity was assessed by Nanodrop and RNA quality was assessed by Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using Ambion kit- Illumina TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
DR 4
|
| Data processing |
Data was processed in R by log2 transformation and z-normalization
|
| |
|
| Submission date |
Sep 19, 2012 |
| Last update date |
Nov 08, 2013 |
| Contact name |
Wilson Chun Yim Fok |
| E-mail(s) |
wilson.fok@wustl.edu
|
| Organization name |
Washington University in St. Louis
|
| Department |
Hematology
|
| Street address |
660 S Euclid Ave
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE40977 |
Combined treatment of rapamycin and dietary restriction has a larger effect on the transcriptome and metabolome of liver |
|
