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Sample GSM1006353 Query DataSets for GSM1006353
Status Public on Jul 03, 2013
Title CNGA3 replicate-3
Sample type RNA
 
Source name retina
Organism Mus musculus
Characteristics genotype: CNGA3-/-/Nrl-/-
age: postnatal day 30
tissue: retina
Growth protocol Samples were obtained from CNGA3-/-/Nrl-/-, CNGB3-/-/Nrl-/-, and Nrl-/- mouse retinas
Extracted molecule total RNA
Extraction protocol Retinas from each animal were pooled and stored in Trizol. Five mice at postnatal 30 days were used in each group. At the time of extraction, the retinas were homogenized and total RNA was isolated using a PureLinkTM RNA Mini Kit (Invitrogen, Carlsbad, CA) following the manufacturer's instructions.
Label biotin
Label protocol RNA samples were labeled using Illumina Total Prep RNA Amplification Kit according to the manufacturer’s directions
 
Hybridization protocol Samples were hybridized to Illumina mouse ref-8 version 2 microarrays and processed according to manufactgurer's directions.
Scan protocol Microarrays were scanned on an Illumina iScan scanner.
Data processing Data from the scanner was obtained with GenomeStudio software (Illumina) and quantile normalized (Matlab).
 
Submission date Sep 19, 2012
Last update date Jul 03, 2013
Contact name Bart Frank
E-mail(s) frank@omrf.org
Organization name OMRF
Street address 825 NE 13 St
City Oklahoma City
State/province OK
ZIP/Postal code 73103
Country USA
 
Platform ID GPL6885
Series (1)
GSE40982 Gene expression in mouse retinas with cone cyclic nucleotide gated channel deficiencies

Data table header descriptions
ID_REF
VALUE log2 transformed, quantile normalized signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 11.64567854 0
ILMN_2721178 9.901912091 0
ILMN_3033922 10.50844272 0
ILMN_3092673 12.52783395 0
ILMN_2816356 7.354193481 0.002506266
ILMN_2808939 9.881124741 0
ILMN_2634564 8.458946923 0
ILMN_2737647 7.057129999 0.4448622
ILMN_2734484 9.827081572 0
ILMN_2952292 7.762605765 0
ILMN_2699078 7.021861375 0.6240602
ILMN_1213681 8.644840974 0
ILMN_2735413 7.062093426 0.4185464
ILMN_2735415 7.02850123 0.5927318
ILMN_2891688 10.49888929 0
ILMN_2637698 10.37464149 0
ILMN_2674228 8.881334861 0
ILMN_2601546 7.052133927 0.4749373
ILMN_1230831 7.302806022 0.003759399
ILMN_2848071 7.220623972 0.02631579

Total number of rows: 25697

Table truncated, full table size 796 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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