|
| Status |
Public on Feb 28, 2013 |
| Title |
Control mouse liver, 1.5X cDNA input, replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Whole liver, not-exposed, archival FFPE
|
| Organism |
Mus musculus |
| Characteristics |
age: 42 days
strain: B6C3F1
gender: male
organ: liver
exposure: none
cdna input: 1.5X
|
| Treatment protocol |
Mice were exposed to PB in drinking water (or drinking water alone) for ~4 weeks
|
| Growth protocol |
16-year-old FFPE liver tissue blocks were used
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen FFPE Rneasy kit; cDNA was synthesized using the WTA2 kit (Sigma)
|
| Label |
Cy3
|
| Label protocol |
cDNA was labeled using the KREAtech ULS genomic DNA labeling kit (Agilent)
|
| |
|
| Hybridization protocol |
1X (samples 1-8) or 1.5X (samples 9-16) cDNA was blocked with Agilent CGH-block and GE-blocking agent and hybridized to Agilent SurePrint GE 8x60 mouse microarrays for 17 hours at 65C at 20rpm
|
| Scan protocol |
Arrays were washed and scanned on an Agilent G2505B Scanner according to manufacturer’s recommendations.
|
| Description |
Pos_2_3_Sample_37
|
| Data processing |
Agilent FE software, version 11 (one color protocol: GE1_1100_Jul11, or two-color protocol: GE2_1100_Jul11).
|
| |
|
| Submission date |
Sep 19, 2012 |
| Last update date |
Feb 28, 2013 |
| Contact name |
Anna Francina Jackson |
| E-mail(s) |
francina.jackson@hc-sc.gc.ca
|
| Organization name |
Health Canada
|
| Department |
Environmental Health Center
|
| Lab |
Dr. Carole Yauk
|
| Street address |
50 Colombine Drive, Tunney's Pasture
|
| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1A 0K9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE40990 |
Preparation of archival formalin-fixed paraffin-embedded mouse liver samples for use with the Agilent gene expression microarray platform |
|
